Relationship between interferon-α conformation and its anti-viral activity determined by circular dichroism and flow cytometry / 生物工程学报
Chinese Journal of Biotechnology
; (12): 1651-1659, 2015.
Article
en Zh
| WPRIM
| ID: wpr-240546
Biblioteca responsable:
WPRO
ABSTRACT
The relationship between the conformation of interferon-α (IFN-α) and its anti-viral activity were analyzed by circular dichroism (CD) and flow cytometry (FCM) techniques. The recombinant human IFN-α (rIFN-α2b and rIFN-α2a) were used. CD spectra from 190 nm to 240 nm indicated that two the IFN-α showed stable secondary structure at 65 degrees C, but unstable when the temperature was above 65 degrees C, and the change was irreversible. FCM data of the anti-viral activity of IFN-α indicated that the change of its secondary structures partly weakened its anti-viral activity. The rIFN-α2b and rIFN-α2a showed the same phenomenon. These data indicated that the conformation of IFN-α is one of the factors to influence its anti-viral activity and the combination of CD and FCM is a good method to analyze the relationship between the conformation of protein drugs and their biological activities in single cell level.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Antivirales
/
Proteínas Recombinantes
/
Química
/
Dicroismo Circular
/
Interferón-alfa
/
Estructura Secundaria de Proteína
/
Citometría de Flujo
Límite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Año:
2015
Tipo del documento:
Article