In vitro study of the effects of CDA-II combined with cAMP on apoptosis induction in retinoic acid resistant acute promyelocytic leukemia cells / 中华血液学杂志
Chinese Journal of Hematology
; (12): 603-606, 2008.
Article
en Zh
| WPRIM
| ID: wpr-239975
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of CDA-II alone or combined with cAMP on the retinoic acid (RA)-resistant acute promyelocytic leukemia (APL) cells.</p><p><b>METHODS</b>The RA-resistant cell line NB4-R2 was used as an in vitro model and treated with CDA-II alone or in combination with cAMP. Cell apoptosis was assessed by morphology observation, distribution of cellular DNA contents and sub-G1 cell population. The level of Bcl-2 was detected by flow cytometry, DNA "ladder" was detected by agarose-electrophoresis.</p><p><b>RESULTS</b>CDA-II could induce NB4-R2 cell apoptosis through decreasing the level of cellular anti-apoptotic protein Bcl-2. cAMP could significantly enhance the role of CDA-II. Bcl-2 positive cell rates decreased to (15.1 +/- 4.8)% and (7.3 +/- 2.9)% in NB4-R2 cells treated with 1 mg/ml CDA-II plus 100 micromol/L cAMP for 48 h and 72 h, respectively. While 100 micromol/L of cAMP could decrease Bcl-2 positive NB4-R2 cells from (92.0 +/- 0.6)% to (75.3 +/- 2.0)%.</p><p><b>CONCLUSIONS</b>CDA-II combined with cAMP could exert potent apoptotic effect on RA-resistant APL cells.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Patología
/
Péptidos
/
Farmacología
/
Fenilacetatos
/
Tretinoina
/
Leucemia Promielocítica Aguda
/
Células Cultivadas
/
Apoptosis
/
AMP Cíclico
/
Proteínas Proto-Oncogénicas c-bcl-2
Límite:
Animals
/
Humans
Idioma:
Zh
Revista:
Chinese Journal of Hematology
Año:
2008
Tipo del documento:
Article