Effect of anacardic acid, a Hsp90 inhibitor, on proliferation, invasion and migration of breast cancer MDA-MB-231 cells / 南方医科大学学报
Journal of Southern Medical University
; (12): 355-359, 2015.
Article
en Zh
| WPRIM
| ID: wpr-239178
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effect of the Hsp90 inhibitor anacardic acid on cell proliferation, invasion and migration of breast cancer MDA-MB-231 cells.</p><p><b>METHODS</b>The inhibitory effect of anacardic acid on Hsp90 was assessed with in vitro ATPase inhibition assay and ATP-sepharose binding assay. MTT assay was used to detect the growth inhibition induced by anacardic acid in MDA-MB-231 cells. Transwell assays were used to evaluate MDA-MB-231 cell invasion and migration. Western blotting was performed to assess the effect of anacardic acid in triggering the degradation of MMP-9, TIMP-1, Hsp90, and Hsp70.</p><p><b>RESULTS</b>Anacardic acid exhibited a modest activity of ATPase inhibition with an IC50 value of 82.5 µmol/L. Anacardic acid significantly suppressed the proliferation of MDA-MB-231 cells in a dose-dependent manner (IC50 value of 29.3 µmol/L). Treatment with 12.5, 25, and 50 µmol/L anacardic acid for 36 h caused inhibition of cell invasion by 23.6%, 56.6%, and 67.0% in MDA-MB-231 cells, respectively (P<0.05), and anacardic acid treatment for 24 h inhibited the cell migration by 30.0%, 45.5%, and 77.5%, respectively (P<0.05). Anacardic acid dose-dependently induced MMP-9 degradation, but did not obviously affect Hsp90 or Hsp70 expressions.</p><p><b>CONCLUSION</b>Anacardic acid can significantly inhibit the proliferation, invasion, and migration of MDA-MB-231 cells, the mechanism of which may involve the inhibition of Hsp90 ATPse activity and down-regulation of MMP-9 expression.</p>
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Base de datos:
WPRIM
Asunto principal:
Patología
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Farmacología
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Neoplasias de la Mama
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Regulación hacia Abajo
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Movimiento Celular
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Proteínas HSP90 de Choque Térmico
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Inhibidor Tisular de Metaloproteinasa-1
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Metaloproteinasa 9 de la Matriz
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Línea Celular Tumoral
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Ácidos Anacárdicos
Límite:
Humans
Idioma:
Zh
Revista:
Journal of Southern Medical University
Año:
2015
Tipo del documento:
Article