Construction of recombinant human smad 7 adenoviral vector and its expression in vitro / 生物医学工程学杂志
J. biomed. eng
; Sheng wu yi xue gong cheng xue za zhi;(6): 769-772, 2005.
Article
en Zh
| WPRIM
| ID: wpr-238345
Biblioteca responsable:
WPRO
ABSTRACT
The recombinant human Smad7 adenoviral vector was constructed by direct DNA cloning protocol and then transfected into 293 cells for virus packaging. After amplification and purification, the recombinant adenovirus was used to infect the keloid fibroblasts. The Smad7 mRNA transcription of the infected cells was detected by RT-PCR. The recombinant Adeno-Smad7 was correctly constructed and confirmed by both restriction analysis and PCR analysis. RT-PCR showed the over expression of adenovirus mediated Smad7 mRNA in keloid cells. These results demonstrated that the recombinant Smad7 adenoviral vector can be expressed in cultured cells in vitro, and it may provide a new therapeutic strategy for keloid gene therapy.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Patología
/
Proteínas Recombinantes de Fusión
/
ARN Mensajero
/
Transfección
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Células Cultivadas
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Adenoviridae
/
Proteína smad7
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Fibroblastos
/
Vectores Genéticos
/
Genética
Tipo de estudio:
Guideline
Límite:
Humans
Idioma:
Zh
Revista:
J. biomed. eng
/
Sheng wu yi xue gong cheng xue za zhi
Año:
2005
Tipo del documento:
Article