Quality control for ABO blood group typing of neonatal umbilical cord blood / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 790-792, 2010.
Article
en Zh
| WPRIM
| ID: wpr-237650
Biblioteca responsable:
WPRO
ABSTRACT
This study was aimed to investigate a quality control method for ABO typing of neonatal umbilical cord blood(UCB). The routine serology method was used to identify the ABO type of UCB samples. These samples with questions were further detected by sequence specific primer PCR (PCR-SSP). The results showed that among total of 76120 UCB samples identified by positive ABO typing, there were 78 samples (1 per thousand) which could not be determined. Of these 78 samples, 30 (56.92%) samples with a weak agglutination reaction were excluded by reverse ABO typing. Out of 260 samples in reverse ABO typing, 148 samples were consistent with positive ABO typing, 112 samples (43.08%) were inconsistent with the positive ABO typing. 58 undetermined samples were detected by PCR-SSP. Out of them the genotyping results of 45 samples confirmed the serological typing, the phenotyping results in 3 cases were inconsistent to that of genotyping. 10 cases showed the unconformity between positive and reverse typing, but the genotyping results were fully consistent with the positive typing. In conclusion, positive typing for red cell antigens combined with PCR-SSP is efficient and sensitive for quality control of ABO typing for neonatal UCB.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Control de Calidad
/
Sistema del Grupo Sanguíneo ABO
/
Tipificación y Pruebas Cruzadas Sanguíneas
/
Sangre Fetal
/
Genética
/
Genotipo
/
Métodos
Tipo de estudio:
Prognostic_studies
Límite:
Humans
/
Newborn
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Año:
2010
Tipo del documento:
Article