Deletions and rearrangements of PAX5 gene in B-lineage acute lymphoblastic leukemia / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
; (6): 549-552, 2013.
Article
en Zh
| WPRIM
| ID: wpr-237209
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To determine the frequency paired-box domain 5 (PAX5) gene alterations in B-lineage acute lymphoblastic leukemia (B-ALL) harboring 9p abnormalities and its implication for clinical prognosis.</p><p><b>METHODS</b>Bacterial artificial chromosomes RP11-344B23 and RP11-652D9 encompassing the PAX5 gene were selected. DNA was extracted with conventional method and labeled with fluorescein by nicking transition. Fluorescence in situ hybridization (FISH) was used to determine the rearrangement or deletion of the PAX5 gene in B-ALL harboring chromosome 9p abnormalities. Clinical and laboratory features of patients were analyzed.</p><p><b>RESULTS</b>Fifty cases were analyzed with FISH. Complete deletion was observed in 23 patients (46%), partial deletion was observed in 2 patients (4%), and rearrangement was detected only in 1 case. The total frequency of abnormalities was 52% (26/50). No significant difference was found in clinical features of patients with or without PAX5 rearrangement or deletion.</p><p><b>CONCLUSION</b>The frequency of PAX5 gene alterations in B-ALL harboring 9p abnormalities was 52%. However, no significant difference was found between patients with and without PAX5 alterations.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Cromosomas Humanos Par 9
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Reordenamiento Génico
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Leucemia de Células B
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Enfermedad Aguda
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Eliminación de Secuencia
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Hibridación Fluorescente in Situ
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Factor de Transcripción PAX5
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Genética
Tipo de estudio:
Prognostic_studies
Límite:
Adolescent
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Adult
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Child
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Female
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Humans
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Male
Idioma:
Zh
Revista:
Chinese Journal of Medical Genetics
Año:
2013
Tipo del documento:
Article