Analyses of Streptomyces coelicolor inner membrane proteome by multidimentional protein identification technology / 生物工程学报
Chinese Journal of Biotechnology
; (12): 814-819, 2005.
Article
en Zh
| WPRIM
| ID: wpr-237068
Biblioteca responsable:
WPRO
ABSTRACT
Streptomyces coelicolor is the model species among streptomycetes. Until now, proteomic analyses of S. coelicolor have been conducted using two-dimensional polyacrylamide gel electrophoresis and matrix-assisted laser desorption ionization time-of-flight mass spectrometry method, few integral membrane proteins were identified due to the hydrophobic and low-abundance nature of these proteins. In this work, 154 possible inner membrane proteins from S. coelicolor were identified using high pH-proteinase K sample preparation method and multidimensional protein identification technology, among them 44 are integral membrane proteins containing at least one transmembrane domain, most peptides and their corresponding proteins were identified experimentally for the first time.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Espectrometría de Masas
/
Proteínas Bacterianas
/
Membrana Celular
/
Química
/
Genoma Bacteriano
/
Proteoma
/
Streptomyces coelicolor
/
Genética
/
Proteínas de la Membrana
/
Métodos
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Año:
2005
Tipo del documento:
Article