Genetically modified industrial brewing yeast with high-glutathione and low-diacetyl production / 生物工程学报
Chinese Journal of Biotechnology
; (12): 942-946, 2005.
Article
en Zh
| WPRIM
| ID: wpr-237046
Biblioteca responsable:
WPRO
ABSTRACT
Recombinant plasmid pICG was constructed by replacing the internal fragment of a-acetohydroxyacid synthase (AHAS) gene (ILV2) with a copy of gamma-glutamylcysteine synthetase gene (GSH1) and copper chelatin gene (CUP1) from the industrial brewing yeast strain YSF31. YSF31 was transformed with plasmid pICG linearized by Kpn I and Pst I. A recombinant strain with high-glutathione and low-diacetyl production was selected. The results of fermentation in 100-L bioreactor showed that the lagering time of beer produced for recombinant strain T2 was shortened by 3 days and the shelf life of the beer was prolonged about 50%. It may be more acceptable for the commercial application, as it does not contain foreign DNA.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Acetolactato Sintasa
/
Saccharomyces cerevisiae
/
Cerveza
/
Regulación Fúngica de la Expresión Génica
/
Clonación Molecular
/
Organismos Modificados Genéticamente
/
Proteínas de Saccharomyces cerevisiae
/
Diacetil
/
Fermentación
/
Glutamato-Cisteína Ligasa
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Año:
2005
Tipo del documento:
Article