Expression profile of nasal NK/T cell lymphoma by cDNA microarray / 中华肿瘤杂志
Zhonghua zhong liu za zhi
; (12): 518-522, 2006.
Article
en Zh
| WPRIM
| ID: wpr-236944
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the changes of gene expression profile in nasal NK/T cell lymphoma.</p><p><b>METHODS</b>Total RNA was extracted from the fresh nasal NK/T cell lymphoma tissue and normal lymph node. Fluorescent labeled cDNA was obtained through synthesizing process by reverse transcription. After hybridization in the two identical microarrays consisting of 4096 genes, overexpressed or underexpressed tumor related genes were analyzed.</p><p><b>RESULTS</b>In both experimental group and control group, there were six samples. A total of 365 (8.9%) genes was found to be differentially expressed by a factor of twofold or greater in both of two identical cDNA microarrays, which included oncogenes, tumor supressor genes, cell cycle regulators, apoptotic and antiapoptotic factors, DNA transcription factors, DNA repair and recombination factors, signal transduction genes, protein translation genes, as well as a large number of metabolic genes. Thirty-seven of these genes were found to be differentially expressed by a factor of fourfold or greater. The biochemical functions of these differentially expressed genes were diverse.</p><p><b>CONCLUSION</b>This study demonstrates that many different kinds of genes are possibly involved in the initiation and progression of nasal NK/T lymphoma. cDNA microarray technique is useful in screening cancer gene expression for nasal NK/T lymphoma.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Patología
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Células Asesinas Naturales
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Receptores Inmunológicos
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Regulación Neoplásica de la Expresión Génica
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Neoplasias Nasales
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Linfoma de Células T
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Antígenos CD20
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Análisis de Secuencia por Matrices de Oligonucleótidos
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Perfilación de la Expresión Génica
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Quimiocina CXCL9
Límite:
Humans
Idioma:
Zh
Revista:
Zhonghua zhong liu za zhi
Año:
2006
Tipo del documento:
Article