Construction of eukaryotic expression plasmid of human PRX3 and its expression in HEK-293FT cells / 华中科技大学学报（医学）（英德文版）
Journal of Huazhong University of Science and Technology (Medical Sciences)
; (6): 311-321, 2004.
Article
en En
| WPRIM
| ID: wpr-236536
Biblioteca responsable:
WPRO
ABSTRACT
To construct the eukaryotic expression plasmid of human PRX3 and measure its expression in the HEK-293FT cells, the full-length coding region of human PRX3 was cloned by PCR and inserted into the eukaryotic expression vector pcDNA4-Xpress (A). HEK-293FT cells were transiently transfected with the recombinant plasmid. Western blot and immuofluorescence were used to detect the expression of the fusion protein. In the experiment, restriction analysis identified the construction of the recombinant plasmid and the inserted sequence was identical with that published on GenBank. Western blot and immunofluorescence confirmed the expression of the recombinant protein in transfected HEK-293FT cells. It was concluded that the eukaryotic expression plasmid of human PRX3 was constructed successfully and the recombinant could be expressed efficiently in HEK-293FT cells, which provides a sound basis for the further study on human PRX3.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Peroxidasas
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Plásmidos
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Línea Celular Transformada
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Transfección
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Expresión Génica
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Clonación Molecular
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Biología Celular
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Embrión de Mamíferos
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Células Eucariotas
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Peroxirredoxinas
Límite:
Humans
Idioma:
En
Revista:
Journal of Huazhong University of Science and Technology (Medical Sciences)
Año:
2004
Tipo del documento:
Article