Establishment of hamster- and human-PRNP transgenic mice / 生物医学与环境科学（英文）
Biomed. environ. sci
; Biomed. environ. sci;(12): 608-616, 2011.
Article
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| WPRIM
| ID: wpr-235591
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To create transgenic mice expressing hamster- and human-PRNP as a model for understanding the physiological function and pathology of prion protein (PrP), as well as the mechanism of cross-species transmission of transmissible spongiform encephalopathies (TSEs).</p><p><b>METHODS</b>Hamster and human-PRNP transgenic mice were established by conventional methods. The copy number of integrated PRNP in various mouse lines was mapped by real-time PCR. PRNP mRNA and protein levels were determined by semi-quantitative RT-PCR, real-time RT-PCR, and western blot analysis. Histological analyses of transgenic mice were performed by hematoxylin and eosin (H & E) staining and immunohistochemical (IHC) methods.</p><p><b>RESULTS</b>Integrated PRNP copy number in various mouse lines was 53 (Tg-haPrP1), 18 (Tg-huPrP1), 3 (Tg-huPrP2), and 16 (Tg-huPrP5), respectively. Exogenous PrPs were expressed at both the transcriptional and translational level. Histological assays did not detect any abnormalities in brain or other organs.</p><p><b>CONCLUSION</b>We have established one hamster-PRNP transgenic mouse line and three human-PRNP transgenic mouse lines. These four transgenic mouse lines provide ideal models for additional research.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Especificidad de Órganos
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Plásmidos
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Transcripción Genética
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Priones
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ADN
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Inmunohistoquímica
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Ratones Transgénicos
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Western Blotting
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Enfermedades por Prión
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Modelos Animales de Enfermedad
Límite:
Animals
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Humans
Idioma:
En
Revista:
Biomed. environ. sci
Año:
2011
Tipo del documento:
Article