Construction and secretory expression of beta-galactosidase gene from Lactobacillus bulgaricus in Lactococcus lactis / 生物医学与环境科学（英文）
Biomedical and Environmental Sciences
; (12): 203-209, 2012.
Article
en En
| WPRIM
| ID: wpr-235534
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>This study is to examine the secretion effects of beta-galactosidase in Lactococcus lactis.</p><p><b>METHODS</b>The usp45 and beta-galactosidase genes were cloned and inserted into plasmid pMG36e to obtain the recombinant plasmid pMG36e-usp-lacZ. This recombinant plasmid was transformed into both Escherichia coli DH5alpha and L. lactis MG1363. The enzyme activity, gene sequencing, SDS-PAGE and hereditary stability were assessed and studied.</p><p><b>RESULTS</b>The lacZ gene inserted into plasmids pMG36e-usp-lacZ was 99.37% similar to the GenBank sequence, and SDS-PAGE revealed an evident idio-strap at 116 KDa between L. lactis MG1363/pMG36e-usp-lacZ in both supernatant and cell samples. Beta-Galactosidase activity measured 0.225 U/mL in L. lactis pMG36e-usp-lacZ transformants, and its secretion rate was 10%. The plasmid pMG36e-usp-lacZ appeared more stable in MG1363.</p><p><b>CONCLUSION</b>The authors concluded that these new recombinant bacteria well expressed and secreted beta-galactosidase, indicating that the beta-galactosidase expression system was successfully constructed, and this might provide a new solution for management of lactose intolerance specifically and promote the use of gene-modified organisms as part of the food-grade plasmid in general.</p>
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Base de datos:
WPRIM
Asunto principal:
Plásmidos
/
Secuencia de Bases
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Beta-Galactosidasa
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Cartilla de ADN
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Electroforesis en Gel de Poliacrilamida
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Genética
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Lactobacillus
Idioma:
En
Revista:
Biomedical and Environmental Sciences
Año:
2012
Tipo del documento:
Article