The application of genotyping in the complicated ABO blood group type / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 461-464, 2013.
Article
en Zh
| WPRIM
| ID: wpr-235330
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the application of ABO blood group genotyping in complicated ABO blood group type.</p><p><b>METHODS</b>Ten specimens of complicated ABO blood group were genotyped by sequence specific primer PCR (PCR -SSP), and confirmed by DNA sequencing and alignment. Six hundred and ten blood samples typed by ABO immunoassay were as control of genotyping.</p><p><b>RESULTS</b>Ten cases of complicated blood type were identified by high resolution PCR- SSP as rare ABO blood groups: cis-AB01 (3 cases), B(A)04 (2 cases), cisAB02, B(A)02, Bel03, Bw12 and Ael05, confirmed by DNA sequencing. Genotyping and serotype detected 610 cases ABO blood group were coincident, and the frequency of A, B, AB and O were as 28.69%, 27.54%, 8.2% and 35.57% respectively. According to the genotypes, the highest frequency subgroup was O1 (32.87%), the lowest was A2 (0.66%).</p><p><b>CONCLUSION</b>PCR -SSP could type the ABO blood group accurately, but also the sub-group of blood type. However, special designed high resolution PCR -SSP or DNA sequencing is needed to identify the complicated blood groups.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Sistema del Grupo Sanguíneo ABO
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Tipificación y Pruebas Cruzadas Sanguíneas
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Reacción en Cadena de la Polimerasa
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Análisis de Secuencia de ADN
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Genética
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Genotipo
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Métodos
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Applied Physiology
Año:
2013
Tipo del documento:
Article