Sperm chromosome analysis and preimplantation genetic diagnos is in an infertile male with mosaic trisomy 18 / 中华医学遗传学杂志
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
; (6): 367-370, 2010.
Article
en Zh
| WPRIM
| ID: wpr-234403
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To analyze the numerical aberration rate of X, Y and chromosome 18 in sperms from an oligozoospermic male with mosaic trisomy 18 and to perform preimplantation genetic diagnosis (PGD) for the couple.</p><p><b>METHODS</b>G-banding and fluorescence in situ hybridization (FISH) were performed on metaphase chromosome. Sperm was analyzed in three-color FISH with a probe mixture containing CEP18, CEPY and Tel Xq/Yq. A healthy man with normal semen parameters was used as control.</p><p><b>RESULTS</b>Significant difference in the rates of disomy for chromosome 18 (0.63% vs. 0.16%) and the gonosomes (0.945% vs. 0.35%) and diploidy (0.87% vs. 0.31%) was found in the spermatozoa between the patient and the control. After four embryos were biopsied in one PGD cycle, two embryos with XY1818 and XX1818 were selected for implanting and clinical pregnancy was ongoing.</p><p><b>CONCLUSION</b>Sperm-FISH allows further understanding of aneuploidy rate and accurate genetic counseling. FISHPGD was effective for patient with mosaic trisomy 18.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Oligospermia
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Espermatozoides
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Trisomía
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Cromosomas Humanos Par 18
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Hibridación Fluorescente in Situ
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Diagnóstico Preimplantación
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Cromosomas Humanos Y
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Diagnóstico
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Diploidia
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Análisis de Semen
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
/
Male
Idioma:
Zh
Revista:
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
Año:
2010
Tipo del documento:
Article