Biocompatibility between human adipose-derived mesenchymal stem cells and porcine bone scaffolds / 南方医科大学学报
Journal of Southern Medical University
; (12): 638-641, 2009.
Article
en Zh
| WPRIM
| ID: wpr-233722
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the compatibility between human adipose-derived mesenchymal stem cells and porcine bone scaffolds.</p><p><b>METHODS</b>Porcine bone tissues were co-cultured with adipose-derived mesenchymal stem cells, and the complex was observed under scanning electron microscope. The viability and alkaline phosphatase (ALP) activity of the cells were examined with the cells co-cultured with human bone scaffold as the control.</p><p><b>RESULTS</b>At 4 and 10 days after the co-culture, the adipose-derived mesenchymal stem cells were observed to extend pseudopodia to adhere to the two scaffold materials. MTT assay showed that the cell proliferation on both of the materials increased with time, and the two cell complexes exhibited similar pattern of changes in ALP activity.</p><p><b>CONCLUSION</b>As the seed cells, human adipose-derived mesenchymal stem cells exhibit good comparability with porcine bone scaffold, suggesting their potential of constructing tissue-engineered bone graft.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Porcinos
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Factores de Tiempo
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Huesos
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Ensayo de Materiales
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Microscopía Electrónica de Rastreo
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Tejido Adiposo
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Técnicas de Cocultivo
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Biología Celular
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Proliferación Celular
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Fosfatasa Alcalina
Límite:
Animals
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Humans
Idioma:
Zh
Revista:
Journal of Southern Medical University
Año:
2009
Tipo del documento:
Article