Sustaining effect of gene-transferring hepatic stellate cell strain CFSC/HGF on hepatocytes development / 中华肝脏病杂志
Chinese Journal of Hepatology
; (12): 45-48, 2005.
Article
en Zh
| WPRIM
| ID: wpr-233625
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the sustaining effects of gene-transferring hepatic stellate cell strain CFSC/HGF on the development of hepatocytes.</p><p><b>METHODS</b>A CFSC/HGF strain, expressing HGF steadily and effectively was established by recombined retroviral vector pMSCV-HGF infection. Morphology and ultra structure of hepatocytes, albumin and urea production, as well as ICG uptake and excretion were studied continuously following the hepatocytes cultured on the CFSC/HGF feeder layers. Parallel group of collagen-dependent hepatocytes culturing and hepatocytes culturing on CFSC were also conducted. Semi-quantitative RT-PCR analysis was made to evaluate the expression of HGF receptor c-Met.</p><p><b>RESULTS</b>The hepatocytes cocultured on CFSC/HGF feeder layers had a higher survival rate, and the functions of albumin secretion and urea syntheses and ICG uptake and excretion, were superior to the other two culture methods. The result of RT-PCR indicated that the c-Met expressed on the CFSC/HGF coculturing hepatocytes was up-regulated 2.23 times.</p><p><b>CONCLUSION</b>Gene-transferring hepatic stellate cell strain CFSC/HGF exhibited a remarkable sustaining effect on the hepatocytes development. The up-regulation of c-Met expressed on the surfaces of the hepatocytes induced by CFSC/HGF might play some part in this function.</p>
Texto completo:
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Base de datos:
WPRIM
Asunto principal:
Transfección
/
Células Cultivadas
/
Factor de Crecimiento de Hepatocito
/
Técnicas de Transferencia de Gen
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Ensayo de Unidades Formadoras de Colonias
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Técnicas de Cocultivo
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Proteínas Proto-Oncogénicas c-met
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Hepatocitos
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Biología Celular
/
Proliferación Celular
Límite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Hepatology
Año:
2005
Tipo del documento:
Article