Relationship between shen-deficiency syndrome and polymorphism of angiotensin converting enzyme gene in longevous elders in Bama area of Guangxi municipality / 中国中西医结合杂志
Zhongguo Zhong Xi Yi Jie He Za Zhi
; (12): 259-263, 2010.
Article
en Zh
| WPRIM
| ID: wpr-231545
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the relationship between Shen-deficiency syndrome (SDS) and the insert/deficit (I/D) polymorphism types (PMTs) of angiotensin converting enzyme (ACE) gene in longevous elders in Bama area of Guangxi municipality.</p><p><b>METHODS</b>The investigation on 27 centenarians (Group A), 56 elders of 90-99 years old (Group B) and 114 of 60-89 years old (Group C) was carried out by questionnaire, and the polymorphism was detected using polymerize chain reaction detection, single strand conformation polymorphism (SSCP) analysis, and direct sequencing technique. And the study was controlled by 79 naturally died elders within 60-70 years old.</p><p><b>RESULTS</b>The presenting frequencies of SDS in various PMT were D/D, I/D and I/I in rising order. The frequencies of PMT, I/I, I/D and D/D, were 37.04% (10/27), 33.33% (9/27), and 29.63% (8/27) respectively; the frequency rates of I and D alleles were 53.70% (29/54) and 46.30% (25/54) in Group A. Comparison of frequency of D/D and D allele between groups showed that Group A was significantly different to Group C (P<0.05) and also to the control (P<0.01), while all the frequencies in Group A and B were rather identical, showing insignificant difference (P>0.05). SSCP analysis showed no significant difference in D/D among groups. Outcomes of direct sequencing test further proved the correctness of I/D ACE polymorphism detection.</p><p><b>CONCLUSION</b>SDS is closely correlated with ACE gene polymorphism and life span. Whereas, other multiple factors that influence longevity should also be taken into consideration.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Polimorfismo Genético
/
Genoma Humano
/
China
/
Peptidil-Dipeptidasa A
/
Pueblo Asiatico
/
Alelos
/
Genética
/
Genotipo
/
Longevidad
/
Medicina Tradicional China
Tipo de estudio:
Diagnostic_studies
Límite:
Aged
/
Aged80
/
Humans
País/Región como asunto:
Asia
Idioma:
Zh
Revista:
Zhongguo Zhong Xi Yi Jie He Za Zhi
Año:
2010
Tipo del documento:
Article