Fabrication of alginate microsphere for controlled release and investigation of its release characteristics in vitro / 生物医学工程学杂志
J. biomed. eng
; Sheng wu yi xue gong cheng xue za zhi;(6): 1301-1304, 2007.
Article
en Zh
| WPRIM
| ID: wpr-230698
Biblioteca responsable:
WPRO
ABSTRACT
This study sought to producte alginate sodium microsphere for controlled release bovine serum albumin(BSA) and to investigate the protein release profile of the BSA-alginate sodium microsphere in vitro, which threw some light on the angiogenesis of tissue engineering bone with vascular endothelial growth factor (VEGF) controlled release under stress. The BSA-alginate sodium microsphere was fabricated with W/O emulsification and ion cross-linking method using alginate sodium. The appearance, microsphere diameter and envelopment rate were detected, and the release characteristics of the BSA-alginate sodium microsphere in vitro was investigated. The alginate microsphere was found to be spherical in shape and evenly distributed. Its mean grain diameter was determined to be 230 +/- 60 microm, carrying capacity 80.3 microg/mg and envelopment rate 61%. Smooth controlled release in BSA-alginate sodium microsphere was shown to last more than 2 weeks. Alginate sodium proved an excellent biodegradable material for protein or polypeptide controlled release. The emulsification and ion cross-linking method was noted to be simple; it was propitious to the structural and functional stablility of protein or polypeptide, thus leading to the prolonged efficacious time of the microsphere.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Albúmina Sérica Bovina
/
Portadores de Fármacos
/
Química
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Ácido Glucurónico
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Preparaciones de Acción Retardada
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Alginatos
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Ácidos Hexurónicos
/
Microesferas
Límite:
Animals
Idioma:
Zh
Revista:
J. biomed. eng
/
Sheng wu yi xue gong cheng xue za zhi
Año:
2007
Tipo del documento:
Article