Restoration of Wild-Type p53 by Adenovirus-Mediated Gene Transfer May Enhance the Therapeutic Efficacy of Chemotherapy in Human Ovarian Cancer Cells / 대한부인종양콜포스코피학회잡지
Korean Journal of Gynecologic Oncology and Colposcopy
; : 23-30, 2001.
Article
en Ko
| WPRIM
| ID: wpr-217367
Biblioteca responsable:
WPRO
ABSTRACT
OBJECTIVE: In an effort to develop a more effective therapeutic strategy for ovarian cancer, we examined whether the restoration of the wild-type p53 gene can enhance the therapeutic effect of chemotherapy. METHODS: In this study, Ov-ca-2774 cells, which are known to have p53 point mutation and cisplatin-resistance, were selected and currently used chemotherapeutic agents including cisplatin, carboplatin, paclitaxel, etoposide, topotecan, and doxorubicin were added concurrently or sequentially with adenovirus-mediated p53 gene transfer (Ad5CMV-p53). RESULTS: Transfer of the wild-type p53 cDNA gene into Ov-ca-2774 cells showed 55% cell killing in vitro at a multiplicity of infection (MOI) of 40. Although the combination of carboplatin or paclitaxel followed by p53 gene transfer with an interval of 48 h manifested no enhanced cell killing compared with cells infected with Ad5CMV-p53 alone, the other combinations of chemotherapeutic agents and p53 gene transfer resulted in 15% to 37% further cell killing (P<0.05). Furthermore, p53 gene transfer followed by doxorubicin with an interval of 24 h and concurrent combination of etoposide with p53 gene transfer showed significant difference in cell killing in contrast to the other combination strategies in the respective chemotherapeutic agent exposure groups (P<0.05). CONCLUSION: Our data demonstrated that combination of p53 gene transfer and chemotherapeutic agents had higher cell killing than either of these two modality alone.
Palabras clave
Texto completo:
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Base de datos:
WPRIM
Asunto principal:
Neoplasias Ováricas
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Doxorrubicina
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Carboplatino
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Genes p53
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Cisplatino
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Paclitaxel
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Mutación Puntual
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ADN Complementario
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Topotecan
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Quimioterapia
Límite:
Humans
Idioma:
Ko
Revista:
Korean Journal of Gynecologic Oncology and Colposcopy
Año:
2001
Tipo del documento:
Article