Peptide Mimotopes of Neisseria meningitidis Group B Capsular Polysaccharide
Yonsei Medical Journal
; : 755-758, 2004.
Article
en En
| WPRIM
| ID: wpr-206343
Biblioteca responsable:
WPRO
ABSTRACT
The antigenic similarity between Neisseria meningitidis group B (NMGB) capsular polysaccharide (PS) and human polysialic acid (PSA) has hampered the development of a NMGB PS-based vaccine. But the possibility of a safe vaccine based on NMGB PS has been demonstrated by the existence of the NMGB PS-associated nonautoreactive epitope, which is distinct from those present on human PSA. To obtain peptide mimotopes of NMGB PS, we used HmenB3, a protective and nonautoreactive monoclonal antibody, to screen a phage library with 12 amino acids. We obtained 23 phage clones that bound to HmenB3 but not in the presence of E. coli K1 PS [which is alpha (2-8) -linked PSA like NMGB PS]. The clones contained 3 mimotopes and differed from previously described NMGB PS mimotopes. Immunization with a synthetic peptide of one mimotope elicited anti-NMGB antibodies in BALB/c mice. These mimotopes may be useful in the development of group B meningococcal vaccines.
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Base de datos:
WPRIM
Asunto principal:
Polisacáridos Bacterianos
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Datos de Secuencia Molecular
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Vacunas Bacterianas
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Secuencia de Aminoácidos
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Clonación Molecular
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Neisseria meningitidis Serogrupo B
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Infecciones Meningocócicas
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Ratones Endogámicos BALB C
Límite:
Animals
Idioma:
En
Revista:
Yonsei Medical Journal
Año:
2004
Tipo del documento:
Article