Intravenous KITENIN shRNA Injection Suppresses Hepatic Metastasis and Recurrence of Colon Cancer in an Orthotopic Mouse Model
Journal of Korean Medical Science
; : 1439-1445, 2011.
Article
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| WPRIM
| ID: wpr-197811
Biblioteca responsable:
WPRO
ABSTRACT
KITENIN (KAI1 C-terminal interacting tetraspanin) promotes invasion and metastasis in mouse colon cancer models. In the present study, we evaluated the effects of KITENIN knockdown by intravenous administration of short hairpin RNAs (shRNAs) in an orthotopic mouse colon cancer model, simulating a primary or adjuvant treatment setting. We established orthotopic models for colon cancer using BALB/c mice and firefly luciferase-expressing CT-26 (CT26/Fluc) cells. Tumor progression and response to therapy were monitored by bioluminescence imaging (BLI). In the primary therapy model, treatment with KITENIN shRNA substantially delayed tumor growth (P = 0.028) and reduced the incidence of hepatic metastasis (P = 0.046). In the adjuvant therapy model, KITENIN shRNA significantly reduced the extent of tumor recurrence (P = 0.044). Mice treated with KITENIN shRNA showed a better survival tendency than the control mice (P = 0.074). Our results suggest that shRNA targeting KITENIN has the potential to be an effective tool for the treatment of colon cancer in both adjuvant and metastatic setting.
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Base de datos:
WPRIM
Asunto principal:
Proteínas Portadoras
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Biomarcadores de Tumor
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Neoplasias del Colon
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Progresión de la Enfermedad
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ARN Interferente Pequeño
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Interferencia de ARN
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Línea Celular Tumoral
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Neoplasias Hepáticas
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Proteínas de la Membrana
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Ratones Endogámicos BALB C
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Animals
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En
Revista:
Journal of Korean Medical Science
Año:
2011
Tipo del documento:
Article