Effects of enamel matrix derivatives on the proliferation and the release of growth factors of human periodontal ligament cells / 대한치과보철학회지
The Journal of Korean Academy of Prosthodontics
; : 203-209, 2016.
Article
en En
| WPRIM
| ID: wpr-195081
Biblioteca responsable:
WPRO
ABSTRACT
PURPOSE: Stimulating the proliferation and migration of periodontal ligament cells (PDLCs) has become the main goal of periodontal regeneration. To accomplish this goal, regeneration procedures have been developed, but results have not been predictable. Recently, tissue engineering using enamel matrix derivatives (EMDs) and growth factors has been applied to periodontal regeneration; however, the mechanism of EMDs is largely unknown. The aim of this study was to investigate the effects of EMDs on the proliferation and release of growth factors from PDLCs. MATERIALS AND METHODS: Human PDLCs were removed from individually extracted 3rd molars of healthy young adults, and cultured in the media containing EMDs (Emdogain, Biora, Malmo, Sweden) at concentration of 0, 12.5, 25, 50, 100, and 200 µg/mL each. Cell proliferation and ALP (alka-line phosphatase) activity were measured. The evaluation of growth factors released by PDLCs was also performed by one-way analysis of variance (ANOVA) and Bonferroni's multiple comparison test. RESULTS: Significantly increased proliferation and ALP activity were observed in PDLCs treated with over 25 µg/mL and 50 µg/mL EMDs, respectively. Additionally, treatment of PDLCs with 50 µg/mL resulted in significantly increased release of vascular endothelial growth factor (VEGF) and transforming growth factor (TGF)-β after 24 h and 48 h, respectively. CONCLUSION: EMDs enhance the proliferation and ALP activity of PDLCs, and promote the release of growth factors, including VEGF and TGF-β, from PDLCs. Therefore EMDs could be one of the effective methods for periodontal regeneration.
Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Ligamento Periodontal
/
Regeneración
/
Factores de Crecimiento Transformadores
/
Ingeniería de Tejidos
/
Péptidos y Proteínas de Señalización Intercelular
/
Esmalte Dental
/
Factor A de Crecimiento Endotelial Vascular
/
Proliferación Celular
/
Diente Molar
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
The Journal of Korean Academy of Prosthodontics
Año:
2016
Tipo del documento:
Article