The time-course and RNA interference of TNF-alpha, IL-6, and IL-1beta expression on neuropathic pain induced by L5 spinal nerve transection in rats / 대한마취과학회지
Korean Journal of Anesthesiology
; : 159-169, 2015.
Article
en En
| WPRIM
| ID: wpr-190106
Biblioteca responsable:
WPRO
ABSTRACT
BACKGROUND: The objective of this study was to investigate the time-course of the expression of TNF-alpha, IL-6, and IL-1beta after L5 spinal nerve transection (SNT), and to determine the effect of small interfering RNA (siRNA) targeting these cytokines on neuropathic pain. METHODS: Rats received control siRNA (CON group, n = 80) or a cocktail of siRNAs targeting these cytokines (COCK group, n = 70). The siRNAs were given via intrathecal catheter 1 d prior to SNT, on the operation day, and 1, 2 and 3 d postoperatively. Behavioral tests and levels of the cytokine mRNAs and proteins as well as glial cell activity were following the L5 SNT. RESULTS: In the CON group, TNF-alpha and IL-1beta mRNA levels increased immediately after SNT and remained high for 6 d, while IL-6 transcripts only began to increase after 12 h. TNF-alpha and IL-1beta mRNA levels in the COCK group were lower than in the CON group at all time points (P < 0.05). In the behavioral tests, allodynia and hyperalgesia were significantly lower in the COCK group from 2 d after SNT (P < 0.05). CONCLUSIONS: The time courses of TNF-alpha, IL-6 and IL-1beta mRNA expression after L5 SNT differ. RNA interference may be a method of reducing the development of mechanical allodynia and hyperalgesia in response to nerve injury.
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Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Nervios Espinales
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ARN Mensajero
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Neuroglía
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Citocinas
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Interleucina-6
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Factor de Necrosis Tumoral alfa
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ARN Interferente Pequeño
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Interferencia de ARN
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Catéteres
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Hiperalgesia
Límite:
Animals
Idioma:
En
Revista:
Korean Journal of Anesthesiology
Año:
2015
Tipo del documento:
Article