Growth Suppression of Ovarian Cancer Cells by Interferon-gama / Journal of the Korean Cancer Association, 대한암학회지
Cancer Research and Treatment
; : 236-242, 2001.
Article
en Ko
| WPRIM
| ID: wpr-178538
Biblioteca responsable:
WPRO
ABSTRACT
PURPOSE: Growth regulation of cancer cells very frequently involves tumor suppressor gene p53, Rb and cell cycle regulator, however the molecular biologic mechanisms of growth regulation in ovarian carcinoma cells are not fully defined. To assess the mechanism of growth suppression, we treated IFN-gama in ovarian carcinoma cells. MATERIALS AND METHODS: Growth suppression by treatment of IFN-gama was determined by cell proliferation assay in ovarian carcinoma cell lines. Apoptosis was determined by DNA fragmentation assay and electron microscopy. Molecular mechanism of the apoptosis in ovarian carcinoma cell by IFN-gama was further analyzed by the western blot. RESULTS: We found that IFN-gama had remarkable growth- suppressive effects in PA-1 and A2774 ovarian carcinoma cells in a time-dependent manner. Apoptosis was observed in PA-1 and A2774 cell following treatment of IFN- gama by DNA fragmentation assay and EM. The expression of IRF-1 protein from A2774 and PA-1 cell extracts was elevated by increasing the concentration of IFN-gama. IFN-gama caused an increased expression of the important apoptosis-related gene, ICE (interleukin-1beta-converting enzyme) protein in A2774 and PA-1. CONCLUSION: The coordinate induction of IRF-1 and ICE by IFN-gama in ovarian carcinoma cells suggests a functional relationship between these proteins in programmed cell death. The significance of this study is the molecular biologic background of IFN-gama considered as an alternative treatment trial of ovarian cancers.
Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Neoplasias Ováricas
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Microscopía Electrónica
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Ciclo Celular
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Extractos Celulares
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Línea Celular
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Western Blotting
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Genes Supresores de Tumor
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Muerte Celular
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Apoptosis
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Proliferación Celular
Idioma:
Ko
Revista:
Cancer Research and Treatment
Año:
2001
Tipo del documento:
Article