Salinomycin inhibited cell proliferation and induced apoptosis in human uterine leiomyoma cells
Obstetrics & Gynecology Science
; : 501-506, 2014.
Article
en En
| WPRIM
| ID: wpr-17030
Biblioteca responsable:
WPRO
ABSTRACT
OBJECTIVE: The aim of this study was to investigate the anti-proliferative effect of the salinomycin in cell proliferation and apoptosis in primary cultured human uterine leiomyoma cells. METHODS: Cell viability was measured by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Caspase-3 activity assay and DNA fragmentation assay were performed to determine the effect of apoptosis. The expression of apoptosis regulatory-related proteins was evaluated by western blot. RESULTS: The cell viability and proliferation of uterine leiomyoma cells were significantly reduced by salinomycin treatment in a dose-dependent manner. DNA fragmentation assay results showed apoptotic cell death after salinomycin incubation. Salinomycin activated caspase-3, -8, and -9, causing apoptosis in uterine leiomyoma cells. Down-regulation of Bcl-2, XIAP, and FLIP with a concomitant increase in Bax, Fas, and DR5 were observed. CONCLUSION: These results provided the first evidence that salinomycin induce both intrinsic and extrinsic apoptosis. Therefore, salinomycin may be a promising chemopreventive and therapeutic agent against human uterine leiomyoma.
Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Regulación hacia Abajo
/
Supervivencia Celular
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Western Blotting
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Muerte Celular
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Apoptosis
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Proliferación Celular
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Caspasa 3
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Fragmentación del ADN
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Leiomioma
Límite:
Humans
Idioma:
En
Revista:
Obstetrics & Gynecology Science
Año:
2014
Tipo del documento:
Article