Improvement of pregnancy rate by intrauterine administration of dexamethasone and recombinant human leukemia inhibitory factor at the time of embryo transfer in cattle
Journal of Veterinary Science
; : 569-576, 2016.
Article
en En
| WPRIM
| ID: wpr-167760
Biblioteca responsable:
WPRO
ABSTRACT
Bovine embryos (day 5) were cultured to day 10 with or without 100 ng/mL PGF2α in medium supplemented with control; 100 nM Dex; 1,000 U/mL recombinant human leukemia inhibitory factor (rhLIF); or Dex+rhLIF. Although the rates to development to the blastocyst were not significantly different among groups, the hatching rate after additional culture with Dex +/or rhLIF was significantly higher in all supplemented groups than the control (p < 0.05). In the presence of PGF2α, the hatching rate was significantly restored in all supplemented groups relative to the group treated with only PGF2α and the control (p < 0.05). Embryo transfer (ET) was performed with blastocysts (day 7). PGF2α levels of control recipient cows were significantly higher in the circulatory blood samples collected 60 min after ET than in samples collected 60 min before ET (p < 0.005), and were decreased in cows injected with loading medium supplemented with Dex+rhLIF (p < 0.005). Pregnancy rate was significantly higher in the ET group that received supplemented embryo-loading medium than in the non-supplemented control (p < 0.05). The intrauterine administration of Dex and rhLIF at ET prevented increased PGF2α in circulatory blood and resulted in enhanced pregnancy rate.
Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Prostaglandinas F
/
Blastocisto
/
Dexametasona
/
Leucemia
/
Fertilización In Vitro
/
Índice de Embarazo
/
Transferencia de Embrión
/
Estructuras Embrionarias
/
Factor Inhibidor de Leucemia
Límite:
Animals
/
Humans
/
Pregnancy
Idioma:
En
Revista:
Journal of Veterinary Science
Año:
2016
Tipo del documento:
Article