A Study the Procurement of HLA Class I Typing Trays Using Gushed Out Blood During Placental Delivery
Journal of the Korean Society for Microbiology
; : 53-60, 1999.
Article
en Ko
| WPRIM
| ID: wpr-163010
Biblioteca responsable:
WPRO
ABSTRACT
BACKGROUND: Microlymphocytotoxicity test is most widely used for HLA Class l typing but almost all laboratories depend on imported HLA Class 1 typing trays. Matching criteria for the selection of HLA- matched platelets to treat platelet refractoriness is not as strict as for bone marrow transplantation. Therefore, with the acquisition of various antisera against high frequency HLA antigens, self-made HLA typing trays can be used for HLA typing of HLA-matched platelet donors. METHODS: 140 samples obtained during placental delivery were tested for the presence of HLA antibodies against a well-characterized panel of 90 cells. Specificity of HLA antisera were determined by evaluating the correlation coefficient r of the 2 x 2 table, x2 test. Antisera strength was evaluated by the strength index. RESULTS: HLA antibodies were detected in 25 samples by primary screening and 23 samples also showed a positive reaction in secondary screening(16%). Among 23 samples, 1 1 antisera were of reagent grade quality and 7 were monospecific antisera. DISCUSSION: Imported HLA typing trays can be replaced by harvesting HLA antisera against HLA antigens which are relatively common in Koreans through continuous HLA antibody screening using gushed out blood during placental delivery. (Korean J Blood Transfusion 10(1): 53-60, 1999)
Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Donantes de Tejidos
/
Plaquetas
/
Transfusión Sanguínea
/
Prueba de Histocompatibilidad
/
Tamizaje Masivo
/
Sensibilidad y Especificidad
/
Trasplante de Médula Ósea
/
Antígenos HLA
/
Sueros Inmunes
/
Anticuerpos
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
/
Screening_studies
Límite:
Humans
Idioma:
Ko
Revista:
Journal of the Korean Society for Microbiology
Año:
1999
Tipo del documento:
Article