Serum immunoglobulin fused interferon-alpha inhibited tumor growth in athymic mice bearing colon 26 adenocarcinoma cells
Journal of Veterinary Science
; : 45-50, 2008.
Article
en En
| WPRIM
| ID: wpr-15565
Biblioteca responsable:
WPRO
ABSTRACT
Interferon (IFN) has therapeutic potential for a wide range of infectious and proliferative disorders. However, the half-life of IFN is too short to have a stable therapeutic effect. To overcome this problem, serum immunoglobulin has been fused to IFN. In this study, the efficacy of serum immunoglobulin fused INFs (si-IFN1 and si-IFN2) was evaluated on athymic mice bearing colon 26 adenocarcinoma cells. Seven days after the implantation of tumor cells, each group of mice was injected once a week with si-IFN1 and si-IFN2 at two different concentrations (10 x : 30 microgram/kg and 50 x : 150 microgram/kg). A slight anti-tumoral effect was observed in all 10 x groups compared to the control. In the 50 x groups, however, si-IFN1 and si-IFN2 showed significant anti- tumoral effects compared to the control. To gain more information on the mechanisms associated with the decrease of tumor size, a Western blot assay of apoptosis-related molecules was performed. The protein expression of cytochrome c, caspase 9, 6, and 3 were increased by si-IFN1 and si-IFN2. These 2 IFNs also increased the expressions of p53, p21, Bax and Bad. Interestingly, si-IFN1 and si-IFN2 decreased the expression of VEGF-beta. Taken together, serum immunoglobulin fused IFNs increased therapeutic efficacy under current experimental condition.
Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Polietilenglicoles
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Nitrógeno de la Urea Sanguínea
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Proteínas Recombinantes de Fusión
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Inmunoglobulinas
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Adenocarcinoma
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Interferón-alfa
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Alanina Transaminasa
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Relación Dosis-Respuesta a Droga
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Interferón alfa-2
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Ratones Desnudos
Límite:
Animals
Idioma:
En
Revista:
Journal of Veterinary Science
Año:
2008
Tipo del documento:
Article