beta PAK-interacting exchange factor may regulate actin cytoskeleton through interaction with actin
Experimental & Molecular Medicine
; : 582-587, 2004.
Article
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| WPRIM
| ID: wpr-145920
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WPRO
ABSTRACT
p21-activated kinase (PAK)-interacting exchange factor (PIX) is known to be involved in regulation of Cdc42/Rac GTPases and PAK activity. PIX binds to the proline-rich region of PAK, and regulates biological events through activation of Cdc42/Rac GTPase. To further investigate the role of PIX we produced monoclonal antibodies (Mab) against beta PAK. Three clones; N-C6 against N-terminal half and C-A3 and C-B7 against C- terminal half of beta PAK were generated and characterized. N-C6 Mab detected beta PAK as a major band in most cell lines. C-A3 Mab recognizes GIT-binding domain (GBD), but it does not interfere with GIT binding to beta PAK. Using C-A3 Mab possible beta PAK interaction with actin in PC12 cells was examined. beta PAK Mab (C-A3) specifically precipitated actin of the PC12 cell lysates whereas actin Mab failed to immunoprecpitate beta PAK. Co-sedimentation of PC12 cell lysates with the polymerized F-actin resulted in the recovery of most of beta PAK in the cell lysates. These results suggest that beta PAK may not interact with soluble actin but with polymerized F-actin and revealed that beta PAK constitutes a functional complex with actin. These data indicate real usefulness of the beta PAK Mab in the study of beta PAK role(s) in regulation of actin cyoskeleton.
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Base de datos:
WPRIM
Asunto principal:
Citoesqueleto de Actina
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Actinas
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Estructura Terciaria de Proteína
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Mapeo Epitopo
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Proteínas de Ciclo Celular
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Factores de Intercambio de Guanina Nucleótido
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Proteínas del Citoesqueleto
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Línea Celular Tumoral
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Inmunoprecipitación
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Anticuerpos Monoclonales
Límite:
Animals
Idioma:
En
Revista:
Experimental & Molecular Medicine
Año:
2004
Tipo del documento:
Article