Alterations of Colonic Contractility in an Interleukin-10 Knockout Mouse Model of Inflammatory Bowel Disease
Journal of Neurogastroenterology and Motility
; : 51-61, 2015.
Article
en En
| WPRIM
| ID: wpr-14536
Biblioteca responsable:
WPRO
ABSTRACT
BACKGROUND/AIMS: Inflammatory bowel disease is commonly accompanied by colonic dysmotility and causes changes in intestinal smooth muscle contractility. In this study, colonic smooth muscle contractility in a chronic inflammatory condition was investigated using smooth muscle tissues prepared from interleukin-10 knockout (IL-10(-/-)) mice. METHODS: Prepared smooth muscle sections were placed in an organ bath system. Cholinergic and nitrergic neuronal responses were observed using carbachol and electrical field stimulation with L-NG-nitroarginine methyl ester (L-NAME). The expression of interstitial cells of Cajal (ICC) networks, muscarinic receptors, neuronal nitric oxide synthase (nNOS) and inducible nitric oxide synthase (iNOS) was observed via immunofluorescent staining. RESULTS: The spontaneous contractility and expression of ICC networks in the proximal and distal colon was significantly decreased in IL-10(-/-) mice compared to IL-10(+/+) mice. The contractility in response to carbachol was significantly decreased in the proximal colon of IL-10(-/-) mice compared to IL-10(+/+) mice, but no significant difference was found in the distal colon. In addition, the expression of muscarinic receptor type 2 was reduced in the proximal colon of IL-10(-/-) mice. The nictric oxide-mediated relaxation after electrical field stimulation was significantly decreased in the proximal and distal colon of IL-10(-/-) mice. In inflamed colon, the expression of nNOS decreased, whereas the expression of iNOS increased. CONCLUSIONS: These results suggest that damage to the ICC network and NOS system in the proximal and distal colon, as well as damage to the smooth muscle cholinergic receptor in the proximal colon may play an important role in the dysmotility of the inflamed colon.
Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Relajación
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Baños
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Carbacol
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Enfermedades Inflamatorias del Intestino
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Receptores Muscarínicos
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Interleucina-10
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Ratones Noqueados
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Colon
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Neuronas Nitrérgicas
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Óxido Nítrico Sintasa de Tipo I
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Journal of Neurogastroenterology and Motility
Año:
2015
Tipo del documento:
Article