Effect of Decursin on the Expression of beta-Catenin and Matrix Metalloproteinase-7 in Prostate Cancer Cell Lines / 대한비뇨기과학회지
Korean Journal of Urology
; : 81-88, 2009.
Article
en Ko
| WPRIM
| ID: wpr-123148
Biblioteca responsable:
WPRO
ABSTRACT
PURPOSE: Alterations in the Wnt/beta-catenin pathway are associated with the development and progression of human prostate cancer. Decursin can attenuate the Wnt/beta-catenin pathway. We investigated the relationship between the Wnt/beta-catenin pathway and decursin in prostate cancer cells. MATERIALS AND METHODS: PC-3 and LNCaP cell lines were used. Cell viability was measured with methyl-thiazole tetrazolium bromide (MTT) assays, and cell apoptosis analysis was performed by FACScan. The amount of beta-catenin protein after treatment with decursin was measured by Western blot analysis. Expression of MMP-7 mRNA was detected by real-time polymerase chain reaction (RT-PCR). RESULTS: Death and apoptosis were increased after treatment with decursin 0.5-100 micrometer in PC-3 and LNCaP cells. This was revealed dose and time-dependent increase of cancer cell death on 24, 48 and 72 hours. FACScan showed an increment of apoptosis on 24, 48 hours. Expression of intracellular beta-catenin protein was decreased dose-dependently in both of prostate cancer cell lines. Decursin reduced MMP-7 mRNA expression on 6, 12, 24, 48 hours dose-dependently. CONCLUSIONS: Decursin affects the viability of prostate cancer cells. Increased cancer cell death was associated with increased apoptosis. This study suggests that decursin may play a role in the treatment of prostate cancer.
Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Próstata
/
Neoplasias de la Próstata
/
Benzopiranos
/
Butiratos
/
ARN Mensajero
/
Línea Celular
/
Supervivencia Celular
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Western Blotting
/
Muerte Celular
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Apoptosis
Límite:
Humans
Idioma:
Ko
Revista:
Korean Journal of Urology
Año:
2009
Tipo del documento:
Article