Procurement of HLA Class I Antisera from Multiparous Blood Donors / 대한임상병리학회지
Korean Journal of Clinical Pathology
; : 630-633, 1998.
Article
en Ko
| WPRIM
| ID: wpr-117430
Biblioteca responsable:
WPRO
ABSTRACT
BACKGROUND: HLA antisera are procured mainly from placental blood or blood of multiparous women. The latter has a merit that a large volume of antisera could be obtained, once the antisera are found to be of good quality. METHODS: A total of 1,437 multiparous blood donors were screened for the presence of anti- HLA antibodies. After the first screening with 20 panel cells, initially reactive sera were re- screened with 30 panel cells. RESULTS: Of 1,437 sera, 50 sera (3.5%) were reactive to both the first and the second screening panel cells. Among 50 sera, 25 (50.0%) sera could be assigned for their antibody specificity with r value of 0.8 or more. Only 14 samples (1.0%) showed reactivity to two or more panels with same antigen specificity and strength index of 80% or more. Four donors repeatedly donated blood with specificities of A24, A26, B7, and B7+B40, respectively. CONCLUSIONS: Screening of HLA class I antibodies in multiparous blood donors showed that HLA antisera of good quality could be obtained in about 1% of the donors in Korea.
Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Donantes de Tejidos
/
Donantes de Sangre
/
Tamizaje Masivo
/
Sensibilidad y Especificidad
/
Sueros Inmunes
/
Corea (Geográfico)
/
Anticuerpos
/
Especificidad de Anticuerpos
Tipo de estudio:
Diagnostic_studies
/
Screening_studies
Límite:
Female
/
Humans
País/Región como asunto:
Asia
Idioma:
Ko
Revista:
Korean Journal of Clinical Pathology
Año:
1998
Tipo del documento:
Article