Expression of Heat Shock Protein 60 kDa Is Upregulated in Cervical Cancer
Yonsei Medical Journal
; : 399-406, 2009.
Article
en En
| WPRIM
| ID: wpr-110995
Biblioteca responsable:
WPRO
ABSTRACT
PURPOSE: Cervical cancer caused by the human papilloma virus (HPV) continues to be the cause of yearly death among women. However, it is a curable disease when diagnosed at an early stage. Recently, several researches have reported that heat shock protein (HSP) 60, a chaperone protein of molecular weight of 60 kDa, is involved in carcinogenesis and apoptosis. In order to evaluate the prognostic significance of HSP60 in cervical cancer, we examined differences in the HSP60 expression between cervical cancer and normal tissues in women. MATERIALS AND METHODS: Tissue samples were collected from 20 cervical cancer patients and 20 normal controls. HSP60 expression of cervical cancer and normal tissues were verified by the 2D gel proteomics, semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot analyses. RESULTS: In 2D proteomic analysis, an increase of HSP60 expression was detected in cervical cancer tissues and confirmed by Western blot analysis (p < 0.05). However, messenger RNA (mRNA) levels of HSP60 did not display any significant differences between cervical cancer and normal tissues. CONCLUSION: These results suggest that HSP60 may be involved in the development of cervical cancer and have profound biological and prognostic significance.
Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Electroforesis en Gel Bidimensional
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Neoplasias del Cuello Uterino
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Western Blotting
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Chaperonina 60
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Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
Límite:
Adult
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Aged
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Female
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Humans
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Male
Idioma:
En
Revista:
Yonsei Medical Journal
Año:
2009
Tipo del documento:
Article