Asian Dust Particles Induce TGF-beta1 via Reactive Oxygen Species in Bronchial Epithelial Cells / 결핵및호흡기질환
Tuberculosis and Respiratory Diseases
; : 84-92, 2012.
Article
en En
| WPRIM
| ID: wpr-105221
Biblioteca responsable:
WPRO
ABSTRACT
BACKGROUND: Asian dust storms can be transported across eastern Asia. In vitro, Asian dust particle-induced inflammation and enhancement of the allergic reaction have been observed. However, the fibrotic effects of Asian dust particles are not clear. Production of transforming growth factor beta1 (TGF-beta1) and fibronectin were investigated in the bronchial epithelial cells after exposure to Asian dust particulate matter (AD-PM10). METHODS: During Asian dust storm periods, air samples were collected. The bronchial epithelial cells were exposed to AD-PM10 with and without the antioxidant, N-acetyl-L-cysteine (NAC). Then TGF-beta1 and fibronectin were detected by Western blotting. The reactive oxygen species (ROS) was detected by the measurement of dicholorodihydrofluorescin (DCF), using a FACScan, and visualized by a confocal microscopy. RESULTS: The expression of TGF-beta1, fibronectin and ROS was high after being exposed to AD-PM10, compared to the control. NAC attenuated both TGF-beta1 and fibronectin expression in the AD-PM10-exposed the bronchial epithelial cells. CONCLUSION: AD-PM10 may have fibrotic potential in the bronchial epithelial cells and the possible mechanism is AD-PM10-induced intracellular ROS.
Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Acetilcisteína
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Western Blotting
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Factor de Crecimiento Transformador beta
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Fibronectinas
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Especies Reactivas de Oxígeno
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Pueblo Asiatico
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Contaminantes Atmosféricos
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Polvo
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Células Epiteliales
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Factor de Crecimiento Transformador beta1
Límite:
Humans
País/Región como asunto:
Asia
Idioma:
En
Revista:
Tuberculosis and Respiratory Diseases
Año:
2012
Tipo del documento:
Article