The expression of KIRREL in gastric cancer and its effect on angiogenesis / 安徽医科大学学报
Acta Universitatis Medicinalis Anhui
; (6): 248-252, 2023.
Article
en Zh
| WPRIM
| ID: wpr-1038386
Biblioteca responsable:
WPRO
ABSTRACT
Objective @#To investigate the expression of Kin of IRRE-like protein 1 (KIRREL) in gastric cancer tissues and further explore its effect on angiogenesis．@*Methods @#Western blot and IHC were used to detect the expression of KIRREL in gastric cancer tissues and adjacent tissues． Human gastric cancer cell lines SNU-5 and AGS were selected to construct KIRREL-interfering and overexpressing stable cell lines by lentiviral infection of gastric cancer cells． The expression of hypoxia-inducible factor 1 α ( HIF-1 α) and vascular endothelial growth factor (VEGF) in the control，null，interfering and overexpression groups were measured by Western blot，and the effect of KIRREL on the tube-forming ability of human umbilical vein endothelial cells (HUVECs) was observed by angiogenesis assay. @*Results@#The expression of KIRREL in gastric cancer tissues was higher than that in adjacent tissues (P＜0. 05) ．After silencing KIRREL，the protein expression of HIF-1α and VEGF was significantly reduced，and the tube length and nodes of HUVECs were also significantly reduced ; the overexpression of KIRREL was the opposite(P＜0. 05) ．@*Conclusion @#KIRREL is overexpressed in gastric cancer tissues and may promote angiogenesis in gastric cancer.
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Base de datos:
WPRIM
Idioma:
Zh
Revista:
Acta Universitatis Medicinalis Anhui
Año:
2023
Tipo del documento:
Article