Changes in glial fibrillary acidic protein expression in different brain regions of rats with traumatic brain inj ury / 安徽医科大学学报
Acta Universitatis Medicinalis Anhui
; (6): 1828-1834, 1840, 2023.
Article
en Zh
| WPRIM
| ID: wpr-1036291
Biblioteca responsable:
WPRO
ABSTRACT
Objective @#To observe the expression of glial fibrillary acidic protein (GFAP) in the brain tissue of rats with traumatic brain injury (TBI) and the differential changes between brain regions.@*Methods @#The TBI model of rats was prepared by the modified Feeney free fall method , and it was randomly divided into the sham⁃operated qPCR were used to observe the morphological changes of activated astrocytes and the expression of GFAP in different brain regions after TBI.@*Results @#Immunohistochemical staining showed that astrocytes were activated in all brain regions on the right side of TBI , and the only brain regions that spilled over to the contralateral side ( left) were cortical region 1 and midbrain regions. RT⁃qPCR results showed that GFAP mRNA was highly expressed in all brain regions on the right side of TBI , affecting the contralateral cortex and midbrain regions. Western blot results showed that GFAP protein was highly expressed in all brain regions on the right side of TBI , and it spread to the contralateral midbrain regions. @*Conclusion @#TBI can cause the activation of astrocytes and increase GFAP expression in the right brain regions , while also affecting the contralateral (left) cortex 1 and midbrain regions.
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Base de datos:
WPRIM
Idioma:
Zh
Revista:
Acta Universitatis Medicinalis Anhui
Año:
2023
Tipo del documento:
Article