Inhibitory effect of miR-133a on liver cancer through tar-geted regulation of G6PD expression / 中国现代普通外科进展
Chinese Journal of Current Advances in General Surgery
; (4): 25-29, 2024.
Article
en Zh
| WPRIM
| ID: wpr-1025408
Biblioteca responsable:
WPRO
ABSTRACT
Objective:To explore if miR-133a is involved in the occurrence and development of hepatocellular carcinoma(HCC)via regulating G6PD.Methods:Bioinformatics analysis predicted the binding sites of miR-133a and G6PD;RT-PCR or western blot was used to assess the expres-sion of miR-133a and G6PD in HCC tissues and the adjacent normal tissues;CCK-8 and flow cy-tometry assays were performed to evaluate the effects of miR-133a/G6PD on cell proliferation,apop-tosis;Fluorescent reporter gene and western blot assays were used to assess the effect of miR-133a on G6PD expression.Results:miR-133a expression was decreased in HCC tissues while G6PD was increased(P0.01);Up-regulation of miR-133a significantly reduced G6PD expression(P＜0.01);up-reg-ulation of miR-133a inhibited cell growth and promoted cell apoptosis(P＜0.05),whereas these effects induced by miR-133a over-expression were all abolished when G6PD was up-regulated(P＜0.01).Conclusion:miR-133a represses the occurrence and development of HCC via targeting G6PD.
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Base de datos:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Current Advances in General Surgery
Año:
2024
Tipo del documento:
Article