Sudan black B reducing the intestinal autofluorescence of rats and its application in immunofluorescence / 解剖学报
Acta Anatomica Sinica
; (6): 450-455, 2020.
Article
en Zh
| WPRIM
| ID: wpr-1015559
Biblioteca responsable:
WPRO
ABSTRACT
Objective To develop a feasible method to reduce the rat intestinal autofluorescence (AF). Methods Five rats were sacrificed. After cryostat section, intestinal AF was investigated by immunofluorescence (IF) before and after Sudan black B (SBB) treatment. Results The AF within the different rat intestinal segments was easily detected in both FITC- and TRITC- channel spectrum, which was not affected by serum blocking. After 0. 3% SBB treatment for 10 minutes, a significant reduction of AF was observed in the rat intestinal tissue. Moreover, the SBB treatment did not affect the expression of CD45 in the rat intestine detected by IF staining, but reduced the AF remarkedly. Conclusion The AF in rat intestinal frozen section can be effectively reduced with 0. 3% SBB treatment for 10 minutes.
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Base de datos:
WPRIM
Idioma:
Zh
Revista:
Acta Anatomica Sinica
Año:
2020
Tipo del documento:
Article