Identification of compounds in Lycii Cortex by UPLC-LTQ-Orbitrap-MS / 中国中药杂志
Zhongguo Zhong Yao Za Zhi
; (24): 4486-4494, 2019.
Article
en Zh
| WPRIM
| ID: wpr-1008218
Biblioteca responsable:
WPRO
ABSTRACT
This experiment was performed to analyze and identify the chemical constituents of Lycii Cortex by UPLC-LTQ-OrbitrapMS. The analysis was performed on a Waters Xbridge Shield RP18( 4. 6 mm×250 mm,5 μm) column with the mobile phase of 0. 1%formic acid( A)-acetonitrile( B) under gradient conditions at a flow rate of 1. 0 m L·min-1 and the temperature maintained at 35 ℃ ．Electrospray ionization ion trap time-off light multistage mass spectrometry was applied for qualitative analysis under positive and negative ion modes. The results indicated that 55 compounds consisted of 39 phenolic amides,6 organic acids,3 cyclic peptides,2 coumarins and 5 others. In conclusion,an UPLC-LTQ-Orbitrap-MS method was established to qualitative analysis of Lycii Cortex in this study,and the fragmentation rules of phenolic amides were summarized,which provides a good foundation for further study of Lycii Cortex.
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Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Fenoles
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Espectrometría de Masas
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Medicamentos Herbarios Chinos
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Cromatografía Líquida de Alta Presión
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Cumarinas
Idioma:
Zh
Revista:
Zhongguo Zhong Yao Za Zhi
Año:
2019
Tipo del documento:
Article