Eukaryotic expression of GP5 and M protein of porcine reproductive and respiratory syndrome virus and immunogenicity evaluation / 生物工程学报
Chinese Journal of Biotechnology
; (12): 4809-4823, 2023.
Article
en Zh
| WPRIM
| ID: wpr-1008060
Biblioteca responsable:
WPRO
ABSTRACT
In order to understand the prevalence and evolution of porcine reproductive and respiratory syndrome virus (PRRSV) in China and to develop subunit vaccine against the epidemic lineage, the genetic evolution analysis of PRRSV strains isolated in China from 2001 to 2021 was performed. The representative strains of the dominant epidemic lineage were selected to optimize the membrane protein GP5 and M nucleotide sequences, which were used, with the interferon and the Fc region of immunoglobulin, to construct the eukaryotic expression plasmids pCDNA3.4-IFNα-GP5-Fc and pCDNA3.4-IFNα-M-Fc. Subsequently, the recombinant proteins IFNα-GP5-Fc and IFNα-M-Fc were expressed by HEK293T eukaryotic expression system. The two recombinant proteins were mixed with ISA206VG adjuvant to immunize weaned piglets. The humoral immunity level was evaluated by ELISA and neutralization test, and the cellular immunity level was detected by ELISPOT test. The results showed that the NADC30-like lineage was the main epidemic lineage in China in recent years, and the combination of IFNα-GP5-Fc and IFNα-M-Fc could induce high levels of antibody and cellular immunity in piglets. This study may facilitate the preparation of a safer and more effective new PRRSV subunit vaccine.
Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Porcinos
/
Proteínas Recombinantes
/
Vacunas Virales
/
Proteínas del Envoltorio Viral
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Virus del Síndrome Respiratorio y Reproductivo Porcino
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Síndrome Respiratorio y de la Reproducción Porcina
/
Vacunas de Subunidad
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Células HEK293
/
Anticuerpos Antivirales
Límite:
Animals
/
Humans
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Año:
2023
Tipo del documento:
Article