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Transcriptomic profiling of cardiac tissues from SARS-CoV-2 patients identifies DNA damage
Arutha Kulasinghe; Ning Liu; Chin Wee Tan; James Monkman; Jane E Sinclair; Dharmesh D Bhuva; David Godbolt; Liuliu Pan; Andy Nam; Habib Sadeghirad; Kei Sato; Gianluigi Li Bassi; Ken O'Byrne; Camila Hartmann; Anna Flavia Ribeiro dos Santos Miggiolaro; Gustavo Lenci Marques; Lidia Zytynski Moura; Derek Richard; Mark N Adams; Lucia Noronha; Cristina Pellegrino Baena; Jacky Suen; Rakesh Arora; Gabrielle T Belz; Kirsty Short; Melissa J Davis; Fernando SF Guimaraes; John F Fraser.
Afiliación
  • Arutha Kulasinghe; The University of Queensland
  • Ning Liu; The Walter and Eliza Hall Institute
  • Chin Wee Tan; The Walter and Eliza Hall Institute
  • James Monkman; The University of Queensland Diamantina Institute
  • Jane E Sinclair; The University of Queensland
  • Dharmesh D Bhuva; The Walter and Eliza Hall Institute
  • David Godbolt; The Prince Charles Hospital
  • Liuliu Pan; Nanostring Technologies
  • Andy Nam; Nanostring Technologies
  • Habib Sadeghirad; The University of Queensland Diamantina Institute
  • Kei Sato; The University of Queensland
  • Gianluigi Li Bassi; University of Queensland
  • Ken O'Byrne; Princess Alexandra Hospital
  • Camila Hartmann; Pontifical Catholic University of Parana
  • Anna Flavia Ribeiro dos Santos Miggiolaro; Pontifical Catholic University of Parana
  • Gustavo Lenci Marques; Pontifical Catholic University of Parana
  • Lidia Zytynski Moura; Pontifical Catholic University of Parana
  • Derek Richard; Queensland University of Technology
  • Mark N Adams; Queensland University of Technology
  • Lucia Noronha; Pontifical Catholic University of Parana
  • Cristina Pellegrino Baena; Pontifical Catholic University of Parana
  • Jacky Suen; The University of Queensland
  • Rakesh Arora; The University of Manitoba
  • Gabrielle T Belz; The University of Queensland
  • Kirsty Short; University of Queensland
  • Melissa J Davis; The Walter and Eliza Hall Institute
  • Fernando SF Guimaraes; The University of Queensland Diamantina Institute
  • John F Fraser; The University of Queensland
Preprint en En | PREPRINT-MEDRXIV | ID: ppmedrxiv-22272732
ABSTRACT
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is known to present with pulmonary and extra-pulmonary organ complications. In comparison with the 2009 pandemic (pH1N1), SARS-CoV-2 infection is likely to lead to more severe disease, with multi-organ effects, including cardiovascular disease. SARS-CoV-2 has been associated with acute and long-term cardiovascular disease, but the molecular changes govern this remain unknown. In this study, we investigated the landscape of cardiac tissues collected at rapid autopsy from SARS-CoV-2, pH1N1, and control patients using targeted spatial transcriptomics approaches. Although SARS-CoV-2 was not detected in cardiac tissue, host transcriptomics showed upregulation of genes associated with DNA damage and repair, heat shock, and M1-like macrophage infiltration in the cardiac tissues of COVID-19 patients. The DNA damage present in the SARS-CoV-2 patient samples, were further confirmed by {gamma}-H2Ax immunohistochemistry. In comparison, pH1N1 showed upregulation of Interferon-stimulated genes (ISGs), in particular interferon and complement pathways, when compared with COVID-19 patients. These data demonstrate the emergence of distinct transcriptomic profiles in cardiac tissues of SARS-CoV-2 and pH1N1 influenza infection supporting the need for a greater understanding of the effects on extra-pulmonary organs, including the cardiovascular system of COVID-19 patients, to delineate the immunopathobiology of SARS-CoV-2 infection, and long term impact on health.
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Texto completo: 1 Colección: 09-preprints Base de datos: PREPRINT-MEDRXIV Idioma: En Año: 2022 Tipo del documento: Preprint
Texto completo
Añadir a Mi BVS
Imprimir
XML
Buscar en Google
Texto completo: 1 Colección: 09-preprints Base de datos: PREPRINT-MEDRXIV Idioma: En Año: 2022 Tipo del documento: Preprint