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Adequacy of Self-Collected Anterior Nares Swabs for SARS-CoV-2 Testing by Grade School Children
Jesse Waggoner; Miriam B. Vos; Erika A. Tyburski; Phuong-Vi Nguyen; Jessica M. Ingersoll; Candace Miller; Jullie Sullivan; Mark Griffiths; Cheryl Stone; Macarthur Benoit; Laura Benedit; Brooke Seitter; Robert Jerris; Joshua M. Levy; Colleen S. Kraft; Sarah Farmer; Amanda Foster; Anna Wood; Adrianna L. Westbrook; Claudia R. Morris; Usha N. Sathian; William Heetderks; Li Li; Kristian Roth; Mary Barcus; Timothy Stenzel; Greg S. Martin; Wilbur A. Lam.
Afiliación
  • Jesse Waggoner; Emory University
  • Miriam B. Vos; Childrens Healthcare of Atlanta
  • Erika A. Tyburski; Georgia Institute of Technology, Atlanta, GA, USA
  • Phuong-Vi Nguyen; Emory University School of Medicine, Atlanta, GA, USA
  • Jessica M. Ingersoll; Emory University School of Medicine, Atlanta, GA, USA
  • Candace Miller; Emory University School of Medicine, Atlanta, GA, USA
  • Jullie Sullivan; Emory University School of Medicine, Atlanta, GA, USA
  • Mark Griffiths; Childrens Healthcare of Atlanta, Atlanta, GA, USA
  • Cheryl Stone; Childrens Healthcare of Atlanta, Atlanta, GA, USA
  • Macarthur Benoit; Childrens Healthcare of Atlanta, Atlanta, GA, USA
  • Laura Benedit; Childrens Healthcare of Atlanta, Atlanta, GA, USA
  • Brooke Seitter; Childrens Healthcare of Atlanta, Atlanta, GA, USA
  • Robert Jerris; Childrens Healthcare of Atlanta, Atlanta, GA, USA
  • Joshua M. Levy; Emory University School of Medicine, Atlanta, GA, USA
  • Colleen S. Kraft; Emory University
  • Sarah Farmer; Georgia Institute of Technology, Atlanta, GA, USA
  • Amanda Foster; Georgia Institute of Technology, Atlanta, GA, USA
  • Anna Wood; Pediatric Biostatistics Core, Department of Pediatrics, Emory University
  • Adrianna L. Westbrook; Pediatric Biostatistics Core, Department of Pediatrics, Emory University
  • Claudia R. Morris; Emory University School of Medicine, Atlanta, GA, USA
  • Usha N. Sathian; Childrens Healthcare of Atlanta, Atlanta, GA, USA
  • William Heetderks; National Institute of Biomedical Imaging and Bioengineering, NIH, Bethesda, MD
  • Li Li; Center for Devices and Radiological Health, U.S. Food & Drug Administration
  • Kristian Roth; Center for Devices and Radiological Health, U.S. Food & Drug Administration
  • Mary Barcus; Center for Devices and Radiological Health, U.S. Food & Drug Administration
  • Timothy Stenzel; Center for Devices and Radiological Health, U.S. Food & Drug Administration
  • Greg S. Martin; Emory University School of Medicine, Atlanta, GA, USA
  • Wilbur A. Lam; Emory University School of Medicine, Atlanta, GA, USA
Preprint en En | PREPRINT-MEDRXIV | ID: ppmedrxiv-22270699
ABSTRACT
BackgroundThe goal of this study was to characterize the ability of school-aged children to self-collect adequate anterior nares (AN) swabs for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) testing. MethodsFrom July to August 2021, 287 children, age 4-14 years-old, were prospectively enrolled in the Atlanta area. Symptomatic (n=197) and asymptomatic (n=90) children watched a short instructional video before providing a self-collected AN specimen. Health care workers (HCWs) then collected a second specimen, and useability was assessed by the child and HCW. Swabs were tested side-by-side for SARS-CoV-2. RNase P RNA detection was investigated as a measure of specimen adequacy. ResultsAmong symptomatic children, 87/196 (44.4%) tested positive for SARS-CoV-2 by both self- and HCW-swab. Two children each were positive by self- or HCW-swab; one child had an invalid HCW-swab. Compared to HCW-swabs, self-collected swabs had 97.8% and 98.1% positive and negative percent agreements, respectively, and SARS-CoV-2 Ct values did not differ significantly between groups. Participants [≤]8 years-old were less likely than those >8 to be rated as correctly completing self-collection, but SARS-CoV-2 detection did not differ. Based on RNase P RNA detection, 270/287 children (94.1%) provided adequate self-swabs versus 277/287 (96.5%) HCW-swabs (p=0.24) with no difference when stratified by age. ConclusionsChildren, aged 4-14 years-old, can provide adequate AN specimens for SARS-CoV-2 detection when presented with age-appropriate instructional material, consisting of a video and a handout, at a single timepoint. These data support the use of self-collected AN swabs among school-age children for SARS-CoV-2 testing.
Licencia
cc_by_nc_nd
Texto completo: 1 Colección: 09-preprints Base de datos: PREPRINT-MEDRXIV Tipo de estudio: Experimental_studies / Rct Idioma: En Año: 2022 Tipo del documento: Preprint
Texto completo: 1 Colección: 09-preprints Base de datos: PREPRINT-MEDRXIV Tipo de estudio: Experimental_studies / Rct Idioma: En Año: 2022 Tipo del documento: Preprint