Your browser doesn't support javascript.
loading
Calibration of Two Validated SARS-CoV-2 Pseudovirus Neutralization Assays for COVID-19 Vaccine Evaluation
Yunda Huang; Oleg Borisov; Jia Jin Kee; Lindsay N. Carpp; Terri Wrin; Suqin Cai; Marcella Sarzotti-Kelsoe; Charlene McDanal; Amanda Eaton; Rolando Pajon; John Hural; Christine M. Posavad; Katherine Gill; Shelly Karuna; Lawrence Corey; M. Juliana McElrath; Peter B. Gilbert; Christos J. Petropoulos; David C. Montefiori.
Afiliación
  • Yunda Huang; Fred Hutchinson Cancer Research Center
  • Oleg Borisov; Biomedical Advanced Research and Development Authority
  • Jia Jin Kee; Fred Hutchinson Cancer Research Center
  • Lindsay N. Carpp; Fred Hutchinson Cancer Research Center
  • Terri Wrin; LabCorp-Monogram Biosciences
  • Suqin Cai; LabCorp-Monogram Biosciences
  • Marcella Sarzotti-Kelsoe; Duke University
  • Charlene McDanal; Duke University
  • Amanda Eaton; Duke University
  • Rolando Pajon; Moderna, Inc.
  • John Hural; Fred Hutchinson Cancer Research Center
  • Christine M. Posavad; Fred Hutchinson Cancer Research Center
  • Katherine Gill; University of Cape Town
  • Shelly Karuna; Fred Hutchinson Cancer Research Center
  • Lawrence Corey; Fred Hutchinson Cancer Research Center
  • M. Juliana McElrath; Fred Hutchinson Cancer Research Center
  • Peter B. Gilbert; Fred Hutchinson Cancer Research Center
  • Christos J. Petropoulos; LabCorp-Monogram Biosciences
  • David C. Montefiori; Duke University
Preprint en En | PREPRINT-MEDRXIV | ID: ppmedrxiv-21263049
Artículo de revista
Un artículo publicado en revista científica está disponible y probablemente es basado en este preprint, por medio del reconocimiento de similitud realizado por una máquina. La confirmación humana aún está pendiente.
Ver artículo de revista
ABSTRACT
Vaccine-induced neutralizing antibodies (nAbs) are key biomarkers considered to be associated with vaccine efficacy. In United States Government-sponsored phase 3 efficacy trials of COVID-19 vaccines, nAbs are measured by two different validated pseudovirus-based SARS-CoV-2 neutralization assays, with each trial using one of the two assays. Here we describe and compare the nAb titers obtained in the two assays. We observe that one assay consistently yielded higher nAb titers than the other when both assays were performed on the World Health Organizations anti-SARS-CoV-2 immunoglobulin International Standard, COVID-19 convalescent sera, and mRNA-1273 vaccinee sera. To overcome the challenge this difference in readout poses in comparing/combining data from the two assays, we evaluate three calibration approaches and show that readouts from the two assays can be calibrated to a common scale. These results may aid decision-making based on data from these assays for the evaluation and licensure of new or adapted COVID-19 vaccines.
Licencia
cc_by_nc_nd
Texto completo: 1 Colección: 09-preprints Base de datos: PREPRINT-MEDRXIV Tipo de estudio: Experimental_studies / Prognostic_studies / Rct Idioma: En Año: 2021 Tipo del documento: Preprint
Texto completo: 1 Colección: 09-preprints Base de datos: PREPRINT-MEDRXIV Tipo de estudio: Experimental_studies / Prognostic_studies / Rct Idioma: En Año: 2021 Tipo del documento: Preprint