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Salivary molecular testing for SARS-CoV-2: simplifying the diagnosis without losing accuracy
Francesca Saluzzo; Paola Mantegani; Valeria Poletti de Chaurand; Federica Cugnata; Patrizia Rovere-Querini; Marta Cilla; Patrizia Erba; Sara Racca; Cristina Tresoldi; Caterina Uberti-Foppa; Clelia Di Serio; Daniela Maria Cirillo.
Afiliación
  • Francesca Saluzzo; Division of Immunology, Transplantation and Infectious Disease, IRCCS Ospedale San Raffaele - Milano (Italy)
  • Paola Mantegani; Division of Immunology, Transplantation and Infectious Disease, IRCCS Ospedale San Raffaele - Milano (Italy),
  • Valeria Poletti de Chaurand; Division of Immunology, Transplantation and Infectious Disease, IRCCS Ospedale San Raffaele - Milano (Italy),
  • Federica Cugnata; CUSSB-University Center for Statistics in the Biomedical Sciences, Vita-Salute San Raffaele University - Milano (Italy)
  • Patrizia Rovere-Querini; IRCCS Ospedale San Raffaele and Vita-Salute San Raffaele University - Milano (Italy)
  • Marta Cilla; IRCCS Ospedale San Raffaele and Vita-Salute San Raffaele University - Milano (Italy)
  • Patrizia Erba; Servizio di Medicina Preventiva, IRCCS Ospedale San Raffaele - Milano (Italy)
  • Sara Racca; Laboratorio Microbiologia/Virologia, SMEL, IRCSS Ospedale San Raffaele- Milano (Italy)
  • Cristina Tresoldi; Molecular Hematology Unit, IRCCS Ospedale San Raffaele- Milano (Italy)
  • Caterina Uberti-Foppa; Division of Infectious Diseases, IRCSS Ospedale San Raffaele and Vita-Salute San Raffaele University- Milano (Italy).
  • Clelia Di Serio; CUSSB-University Center for Statistics in the Biomedical Sciences, Vita-Salute San Raffaele University - Milano (Italy)
  • Daniela Maria Cirillo; Division of Immunology, Transplantation and Infectious Disease, IRCCS Ospedale San Raffaele - Milano (Italy)
Preprint en En | PREPRINT-MEDRXIV | ID: ppmedrxiv-21260706
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ABSTRACT
BackgroundQuantitative RT-PCR on NasoPharyngeal Swab (NPS) is still considered the standard for the diagnosis of SARS-CoV-2 infection, even if saliva has been evaluated in several studies as a possible alternative. The use of point of care (POC) platforms, providing highly specific results performed on saliva could simplify the diagnosis of COVID-19 and contribute to contain the spreading of SARS-CoV-2. MethodsWe assess the sensitivity and specificity of molecular testing performed on saliva in comparison to NPS using two different POC platforms (DiaSorin Simplexa and Cepheid Xpert(R)). NPS and saliva were collected prospectically from asymptomatic health care workers and mildly symptomatic patients. Moreover, the stability of saliva samples after storage at -80{degrees}C for up to 45 days was tested. ResultsThe obtained results in comparison to NPS demonstrated for both DiaSorin Simplexa and Xpert(R) Xpress a specificity of 100% and a sensitivity of 90.24%. The overall agreement between the tests performed on saliva was 98%. A positive correlation in Ct values detected on saliva and on NPS was identified for all the targets shared by the tests in analysis (Orf1ab, E and N2). Both S Ct values and Orf1ab Ct values were not significantly different before and after the freezing in the tested saliva samples. ConclusionThe obtained results demonstrated an overall performance of saliva comparable to NPS, confirming that RT-PCR performed using POCs on saliva could represent a valid public health solution for controlling SARS-CoV-2 pandemic.
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Texto completo: 1 Colección: 09-preprints Base de datos: PREPRINT-MEDRXIV Tipo de estudio: Diagnostic_studies / Experimental_studies Idioma: En Año: 2021 Tipo del documento: Preprint
Texto completo: 1 Colección: 09-preprints Base de datos: PREPRINT-MEDRXIV Tipo de estudio: Diagnostic_studies / Experimental_studies Idioma: En Año: 2021 Tipo del documento: Preprint