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Simply saliva: stability of SARS-CoV-2 detection negates the need for expensive collection devices
Isabel M Ott; Madison S Strine; Anne E Watkins; Maikel Boot; Chaney C Kalinich; Christina A Harden; Chantal B.F. Vogels; Arnau Casanovas-Massana; Adam J Moore; M. Catherine Muenker; Maura Nakahata; Maria Tokuyama; Allison Nelson; John Fournier; Santos Bermejo; Melissa Campbell; Rupak Datta; - the Yale IMPACT Research team; Charles S Dela Cruz; Shelli F Farhadian; Albert I Ko; Akiko Iwasaki; Nathan D Grubaugh; Craig B Wilen; Anne Louise Wyllie.
Afiliación
  • Isabel M Ott; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT 06510, USA
  • Madison S Strine; Department of Laboratory Medicine, Yale University School of Medicine, New Haven, CT, 06510, USA
  • Anne E Watkins; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT 06510, USA
  • Maikel Boot; Department of Microbial Pathogenesis, Yale University School of Medicine, New Haven, CT, 06510, USA
  • Chaney C Kalinich; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT 06510, USA
  • Christina A Harden; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT 06510, USA
  • Chantal B.F. Vogels; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT 06510, USA
  • Arnau Casanovas-Massana; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT 06510, USA
  • Adam J Moore; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT 06510, USA
  • M. Catherine Muenker; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT 06510, USA
  • Maura Nakahata; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT 06510, USA
  • Maria Tokuyama; Department of Immunobiology, Yale School of Medicine, New Haven, CT, 06510, USA
  • Allison Nelson; Department of Internal Medicine, Section of Infectious Diseases, Yale School of Medicine, New Haven, CT, 06510, USA
  • John Fournier; Department of Medicine, Section of Infectious Diseases, Yale School of Medicine, New Haven, CT, 06510, USA
  • Santos Bermejo; Department of Internal Medicine, Section of Pulmonary, Critical Care, and Sleep Medicine, Yale School of Medicine, New Haven, CT, 06510, USA
  • Melissa Campbell; Department of Pediatrics, Division of Infectious Diseases, Yale School of Medicine, New Haven, CT, 06510, USA
  • Rupak Datta; Department of Medicine, Section of Infectious Diseases, Yale School of Medicine, New Haven, CT, 06510, USA
  • - the Yale IMPACT Research team;
  • Charles S Dela Cruz; Department of Internal Medicine, Section of Pulmonary, Critical Care, and Sleep Medicine, Yale School of Medicine, New Haven, CT, 06510, USA
  • Shelli F Farhadian; Department of Medicine, Section of Infectious Diseases, Yale School of Medicine, New Haven, CT, 06510, USA
  • Albert I Ko; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT 06510, USA; Department of Medicine, Section of Infectious Diseases
  • Akiko Iwasaki; Department of Immunobiology, Yale School of Medicine, New Haven, CT, 06510, USA; Howard Hughes Medical Institute, New Haven, CT 06510, USA
  • Nathan D Grubaugh; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT 06510, USA
  • Craig B Wilen; Department of Laboratory Medicine, Yale University School of Medicine, New Haven, CT, 06510, USA
  • Anne Louise Wyllie; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT 06510, USA
Preprint en En | PREPRINT-MEDRXIV | ID: ppmedrxiv-20165233
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ABSTRACT
Most currently approved strategies for the collection of saliva for COVID-19 diagnostics require specialized tubes containing buffers promoted for the stabilization of SARS-CoV-2 RNA and virus inactivation. Yet many of these are expensive, in limited supply, and not necessarily validated specifically for viral RNA. While saliva is a promising sample type as it can be reliably self-collected for the sensitive detection of SARS-CoV-2, the expense and availability of these collection tubes are prohibitive to mass testing efforts. Therefore, we investigated the stability of SARS-CoV-2 RNA and infectious virus detection from saliva without supplementation. We tested RNA stability over extended periods of time (2-25 days) and at temperatures representing at-home storage and elevated temperatures which might be experienced when cold chain transport may be unavailable. We found SARS-CoV-2 RNA in saliva from infected individuals is stable at 4{degrees}C, room temperature ([~]19{degrees}C), and 30{degrees}C for prolonged periods and found limited evidence for viral replication in stored saliva samples. This work demonstrates that expensive saliva collection options involving RNA stabilization and virus inactivation buffers are not always needed, permitting the use of cheaper collection options. Affordable testing methods are urgently needed to meet current testing demands and for continued surveillance in reopening strategies.
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Texto completo: 1 Colección: 09-preprints Base de datos: PREPRINT-MEDRXIV Tipo de estudio: Diagnostic_studies / Prognostic_studies Idioma: En Año: 2020 Tipo del documento: Preprint
Texto completo
Añadir a Mi BVS
Imprimir
XML
Buscar en Google
Texto completo: 1 Colección: 09-preprints Base de datos: PREPRINT-MEDRXIV Tipo de estudio: Diagnostic_studies / Prognostic_studies Idioma: En Año: 2020 Tipo del documento: Preprint