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D614G Spike Mutation Increases SARS CoV-2 Susceptibility to Neutralization.
Drew Weissman; Mohamad-Gabriel Alameh; Tushan de Silva; Paul Collini; Hailey Hornsby; Rebecca Brown; Celia C LaBranche; Robert J Edwards; Laura Sutherland; Sampa Santra; Katayoun Mansouri; Sophie Gobeil; Charlene McDanal; Norbert Pardi; Nick Hengartner; Paulo J.C. Lin; Ying Tam; Pamela A Shaw; Mark G Lewis; Carsten Boesler; Ugur Sahin; Priyamvada Acharya; Barton F Haynes; Bette Korber; David C Montefiori.
Afiliación
  • Drew Weissman; University of Pennsylvania
  • Mohamad-Gabriel Alameh; University of Pennsylvania
  • Tushan de Silva; University of Sheffield
  • Paul Collini; University of Sheffield
  • Hailey Hornsby; University of Sheffield
  • Rebecca Brown; University of Sheffield
  • Celia C LaBranche; Duke University
  • Robert J Edwards; Duke University
  • Laura Sutherland; Duke University
  • Sampa Santra; Harvard University
  • Katayoun Mansouri; Duke University
  • Sophie Gobeil; Duke University
  • Charlene McDanal; Duke University
  • Norbert Pardi; University of Pennsylvania
  • Nick Hengartner; LANL
  • Paulo J.C. Lin; Acuitas Therapeutics
  • Ying Tam; Acuitas Therapeutics
  • Pamela A Shaw; University of Pennsylvania
  • Mark G Lewis; Bioqual
  • Carsten Boesler; BioNTech
  • Ugur Sahin; BioNTech
  • Priyamvada Acharya; Duke University
  • Barton F Haynes; Duke University
  • Bette Korber; Los Alamos National Laboratory
  • David C Montefiori; Duke University
Preprint en En | PREPRINT-MEDRXIV | ID: ppmedrxiv-20159905
Artículo de revista
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ABSTRACT
The SARS-CoV-2 Spike protein acquired a D614G mutation early in the COVID-19 pandemic that appears to confer on the virus greater infectivity and is now the globally dominant form of the virus. Certain of the current vaccines entering phase 3 trials are based on the original D614 form of Spike with the goal of eliciting protective neutralizing antibodies. To determine whether D614G mediates neutralization-escape that could compromise vaccine efficacy, sera from Spike-immunized mice, nonhuman primates and humans were evaluated for neutralization of pseudoviruses bearing either D614 or G614 Spike on their surface. In all cases, the G614 pseudovirus was moderately more susceptible to neutralization. The G614 pseudovirus also was more susceptible to neutralization by monoclonal antibodies against the receptor binding domain and by convalescent sera from people known to be infected with either the D614 or G614 form of the virus. These results indicate that a gain in infectivity provided by D614G came at the cost of making the virus more vulnerable to neutralizing antibodies, and that the mutation is not expected to be an obstacle for current vaccine development.
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Texto completo: 1 Colección: 09-preprints Base de datos: PREPRINT-MEDRXIV Tipo de estudio: Experimental_studies Idioma: En Año: 2020 Tipo del documento: Preprint
Texto completo: 1 Colección: 09-preprints Base de datos: PREPRINT-MEDRXIV Tipo de estudio: Experimental_studies Idioma: En Año: 2020 Tipo del documento: Preprint