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Shared Antigen-specific CD8+ T cell Responses Against the SARS-COV-2 Spike Protein in HLA A*02:01 COVID-19 Participants
William Chour; Alex M Xu; Alphonsus H.C. Ng; Jongchan Choi; Jingyi Xie; Dan Yuan; John K. Lee; Diane C. Delucia; Rick Edmark; Lesley Jones; Thomas M. Schmitt; Mary E. Chaffee; Venkata Duvvuri; Philip D. Greenberg; Kim Murray; Julie Wallick; Heather A. Algren; William R. Berrington; Jason D. Goldman; James R Heath.
Afiliación
  • William Chour; Institute for Systems Biology
  • Alex M Xu; Institute for Systems Biology
  • Alphonsus H.C. Ng; Institute for Systems Biology
  • Jongchan Choi; Institute for Systems Biology
  • Jingyi Xie; Institute for Systems Biology
  • Dan Yuan; Institute for Systems Biology
  • John K. Lee; Fred Hutch Cancer Research Institute
  • Diane C. Delucia; Fred Hutch Cancer Research Center
  • Rick Edmark; Institute for Systems Biology
  • Lesley Jones; Institute for Systems Biology
  • Thomas M. Schmitt; Fred Hutch Cancer Research Institute
  • Mary E. Chaffee; Fred Hutch Cancer Research Center
  • Venkata Duvvuri; Institute for Systems Biology
  • Philip D. Greenberg; Fred Hutch Cancer Research Center
  • Kim Murray; Institute for Systems Biology
  • Julie Wallick; Swedish Medical Center
  • Heather A. Algren; Swedish Medical Center
  • William R. Berrington; Swedish Medical Center
  • Jason D. Goldman; Swedish Medical Center
  • James R Heath; Institute for Systems Biology
Preprint en En | PREPRINT-MEDRXIV | ID: ppmedrxiv-20085779
ABSTRACT
We report here on antigens from the SARS-CoV-2 virus spike protein, that when presented by Class I MHC, can lead to cytotoxic CD8+ T cell anti-viral responses in COVID-19 patients. We present a method in which the SARS-CoV-2 spike protein is converted into a library of peptide antigen-Major Histocompatibility Complexes (pMHCs) as single chain trimers that contain the peptide antigen, the MHC HLA allele subunit, and the {beta}-2 microglobulin subunit. This library is used to detect the evolution of virus-specific T cell populations in four COVID-19 study participants two of which share one HLA allele, and the other two a second HLA allele, at two time points over the initial course of infection. HLA-matched participants exhibit similar virus-specific T cell populations, but very different time-trajectories of those populations. This strategy can be used to track those virus-specific T cell populations over the course of an infection, thus providing deep insight into the SARS-CoV-2 immune system trajectories observed in different COVID-19 patients.
Licencia
cc_by_nc_nd
Texto completo: 1 Colección: 09-preprints Base de datos: PREPRINT-MEDRXIV Tipo de estudio: Prognostic_studies Idioma: En Año: 2020 Tipo del documento: Preprint
Texto completo: 1 Colección: 09-preprints Base de datos: PREPRINT-MEDRXIV Tipo de estudio: Prognostic_studies Idioma: En Año: 2020 Tipo del documento: Preprint