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Within-host evolutionary dynamics and tissue compartmentalization during acute SARS-CoV-2 infection
Mireille Farjo; Katia Koelle; Michael A. Martin; Laura L Gibson; Kimberly KO Walden; Gloria Rendon; Christopher J. Fields; Fadi Alnaji; Nicholas Gallagher; Chun Huai Luo; Heba H. Mostafa; Yukari C Manabe; Andrew Pekosz; Rebecca Lee Smith; David D McManus; Christopher B Brooke.
Afiliación
  • Mireille Farjo; University of Illinois at Urbana-Champaign
  • Katia Koelle; Emory University
  • Michael A. Martin; Emory University
  • Laura L Gibson; University of Massachusetts Medical School
  • Kimberly KO Walden; University of Illinois at Urbana-Champaign
  • Gloria Rendon; University of Illinois at Urbana Champaign
  • Christopher J. Fields; University of Illinois at Urbana Champaign
  • Fadi Alnaji; University of Illinois at Urbana Champaign
  • Nicholas Gallagher; Johns Hopkins University School of Medicine
  • Chun Huai Luo; Johns Hopkins University School of Medicine
  • Heba H. Mostafa; Johns Hopkins University School of Medicine
  • Yukari C Manabe; Johns Hopkins University School of Medicine
  • Andrew Pekosz; Johns Hopkins Bloomberg School of Public Health
  • Rebecca Lee Smith; University of Illinois at Urbana-Champaign
  • David D McManus; University of Massachusetts Medical School
  • Christopher B Brooke; University of Illinois at Urbana-Champaign
Preprint en En | PREPRINT-BIORXIV | ID: ppbiorxiv-497047
ABSTRACT
The global evolution of SARS-CoV-2 depends in part upon the evolutionary dynamics within individual hosts with varying immune histories. To characterize the within-host evolution of acute SARS-CoV-2 infection, we deep sequenced saliva and nasal samples collected daily from immune and unvaccinated individuals early during infection. We show that longitudinal sampling facilitates high-confidence genetic variant detection and reveals evolutionary dynamics missed by less-frequent sampling strategies. Within-host dynamics in both naive and immune individuals appeared largely stochastic; however, we identified clear mutational hotspots within the viral genome, consistent with selection and differing between naive and immune individuals. In rare cases, minor genetic variants emerged to frequencies sufficient for forward transmission. Finally, we detected significant genetic compartmentalization of virus between saliva and nasal swab sample sites in many individuals. Altogether, these data provide a high-resolution profile of within-host SARS-CoV-2 evolutionary dynamics.
Licencia
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Texto completo: 1 Colección: 09-preprints Base de datos: PREPRINT-BIORXIV Tipo de estudio: Prognostic_studies Idioma: En Año: 2022 Tipo del documento: Preprint
Texto completo: 1 Colección: 09-preprints Base de datos: PREPRINT-BIORXIV Tipo de estudio: Prognostic_studies Idioma: En Año: 2022 Tipo del documento: Preprint