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Isolation and Characterization of Cross-Neutralizing Coronavirus Antibodies from COVID-19+ Subjects
Madelein Jennewein; Anna MacCamey; Nicholas Akins; Junli Feng; Leah Homad; Nicholas Hurlburt; Emily Seydoux; Yu-Hsin Wang; Andrew B Stuart; Venkata Vishwanadh Edara; Katharine Floyd; Abigail Vanderheiden; John R. Mascola; Nicole Doria-Rose; Lingshu Wang; Eun Yang; Helen Chu; Jonathan Torres; Gabriel Ozorowski; Andrew Ward; Rachael Whaley; Kristen Cohen; Marie Pancera; Juliana McElrath; Janet A Englund; Andres Finzi; Mehul Suthar; Andrew McGuire; Leonidas Stamatatos.
Afiliación
  • Madelein Jennewein; Fred Hutchinson Cancer Research Center
  • Anna MacCamey; Fred Hutchinson Cancer Research Center
  • Nicholas Akins; Fred Hutchinson Cancer Research Center
  • Junli Feng; Fred Hutchinson Cancer Research Center
  • Leah Homad; Fred Hutchinson Cancer Research Center
  • Nicholas Hurlburt; Fred Hutchinson Cancer Research Center
  • Emily Seydoux; Fred Hutchinson Cancer Research Center
  • Yu-Hsin Wang; Fred Hutchinson Cancer Research Center
  • Andrew B Stuart; Fred Hutchinson Cancer Research Center
  • Venkata Vishwanadh Edara; Emory University
  • Katharine Floyd; Emory University
  • Abigail Vanderheiden; Emory University
  • John R. Mascola; Vaccine Research Center, NIAID, NIH
  • Nicole Doria-Rose; NIH/ VRC
  • Lingshu Wang; VRC/NIAID/NIH
  • Eun Yang; NIH/VRC
  • Helen Chu; University of Washington
  • Jonathan Torres; TSRI
  • Gabriel Ozorowski; Scripps Research Institute
  • Andrew Ward; The Scripps Research Institute
  • Rachael Whaley; Fred Hutchinson Cancer Research Center
  • Kristen Cohen; Fred Hutchinson Cancer Research Center
  • Marie Pancera; Fred Hutchinson Cancer Research Center
  • Juliana McElrath; Fred Hutchinson Cancer Research Center
  • Janet A Englund; University of Washington
  • Andres Finzi; University of Montreal
  • Mehul Suthar; Emory University
  • Andrew McGuire; Fred Hutch
  • Leonidas Stamatatos; Fred Hutchinson Cancer Research Center
Preprint en En | PREPRINT-BIORXIV | ID: ppbiorxiv-436684
Artículo de revista
Un artículo publicado en revista científica está disponible y probablemente es basado en este preprint, por medio del reconocimiento de similitud realizado por una máquina. La confirmación humana aún está pendiente.
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ABSTRACT
SARS-CoV-2 is one of three coronaviruses that have crossed the animal-to-human barrier in the past two decades. The development of a universal human coronavirus vaccine could prevent future pandemics. We characterized 198 antibodies isolated from four COVID19+ subjects and identified 14 SARS-CoV-2 neutralizing antibodies. One targeted the NTD, one recognized an epitope in S2 and twelve bound the RBD. Three anti-RBD neutralizing antibodies cross-neutralized SARS-CoV-1 by effectively blocking binding of both the SARS-CoV-1 and SARS-CoV-2 RBDs to the ACE2 receptor. Using the K18-hACE transgenic mouse model, we demonstrate that the neutralization potency rather than the antibody epitope specificity regulates the in vivo protective potential of anti-SARS-CoV-2 antibodies. The anti-S2 antibody also neutralized SARS-CoV-1 and all four cross-neutralizing antibodies neutralized the B.1.351 mutant strain. Thus, our study reveals that epitopes in S2 can serve as blueprints for the design of immunogens capable of eliciting cross-neutralizing coronavirus antibodies.
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Texto completo: 1 Colección: 09-preprints Base de datos: PREPRINT-BIORXIV Tipo de estudio: Rct Idioma: En Año: 2021 Tipo del documento: Preprint
Texto completo: 1 Colección: 09-preprints Base de datos: PREPRINT-BIORXIV Tipo de estudio: Rct Idioma: En Año: 2021 Tipo del documento: Preprint