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SARS-CoV-2 RBD-Tetanus toxoid conjugate vaccine induces a strong neutralizing immunity in preclinical studies
Yury Valdes-Balbin; Darielys Santana-Mederos; Lauren Quintero; Sonsire Fernandez; Laura Rodriguez; Belinda Sanchez-Ramirez; Rocmira Perez; Claudia Acosta; Yanira Mendez; Manuel G Ricardo; Tays Hernandez; Gretchen Bergado; Franciscary Pi; Annet Valdes; Tania Carmenate; Ubel Ramirez; Reynaldo Oliva; Jean-Pierre Soubal; Raine Garrido; Felix Cardoso; Mario Landys; Mildrey Farinas; Humberto Gonzalez; Juliet Enriquez; Enrique Noa; Anamary Suarez; Cheng Fang; Luis A Espinosa; Yassel Ramos; Luis Javier Gonzalez; Yanet Climent; Gertrudis Rojas; Ernesto Relova-Hernandez; Yanelys Cabrera-Infante; Sum Lai Losada; Tammy Boggiano; Eduardo Ojito; Kalet Leon-Monzon; Fabrizio Chiodo; Francoise Paquet; Guangwu Chen; Daniel G Rivera; Dagmar Garcia-Rivera; Vicente Verez-Bencomo.
Afiliación
  • Yury Valdes-Balbin; Finlay Vaccine Institute, 200 and 21 Street, Havana 11600, Cuba
  • Darielys Santana-Mederos; Finlay Vaccine Institute, 200 and 21 Street, Havana 11600, Cuba
  • Lauren Quintero; Finlay Vaccine Institute, 200 and 21 Street, Havana 11600, Cuba
  • Sonsire Fernandez; Finlay Vaccine Institute, 200 and 21 Street, Havana 11600, Cuba
  • Laura Rodriguez; Finlay Vaccine Institute, 200 and 21 Street, Havana 11600, Cuba
  • Belinda Sanchez-Ramirez; Center of Molecular Immunology, P.O. Box 16040, 216 St. Havana, Cuba
  • Rocmira Perez; Finlay Vaccine Institute, 200 and 21 Street, Havana 11600, Cuba
  • Claudia Acosta; Finlay Vaccine Institute, 200 and 21 Street, Havana 11600, Cuba
  • Yanira Mendez; Laboratory of Synthetic and Biomolecular Chemistry, Faculty of Chemistry, University of Havana, Cuba
  • Manuel G Ricardo; Laboratory of Synthetic and Biomolecular Chemistry, Faculty of Chemistry, University of Havana, Cuba
  • Tays Hernandez; Center of Molecular Immunology, P.O. Box 16040, 216 St. Havana, Cuba
  • Gretchen Bergado; Center of Molecular Immunology, P.O. Box 16040, 216 St. Havana, Cuba
  • Franciscary Pi; Center of Molecular Immunology, P.O. Box 16040, 216 St. Havana, Cuba
  • Annet Valdes; Center of Molecular Immunology, P.O. Box 16040, 216 St. Havana, Cuba
  • Tania Carmenate; Center of Molecular Immunology, P.O. Box 16040, 216 St. Havana, Cuba
  • Ubel Ramirez; Finlay Vaccine Institute, 200 and 21 Street, Havana 11600, Cuba.
  • Reynaldo Oliva; Finlay Vaccine Institute, 200 and 21 Street, Havana 11600, Cuba.
  • Jean-Pierre Soubal; Finlay Vaccine Institute, 200 and 21 Street, Havana 11600, Cuba.
  • Raine Garrido; Finlay Vaccine Institute, 200 and 21 Street, Havana 11600, Cuba.
  • Felix Cardoso; Finlay Vaccine Institute, 200 and 21 Street, Havana 11600, Cuba.
  • Mario Landys; Finlay Vaccine Institute, 200 and 21 Street, Havana 11600, Cuba.
  • Mildrey Farinas; Finlay Vaccine Institute, 200 and 21 Street, Havana 11600, Cuba
  • Humberto Gonzalez; Finlay Vaccine Institute, 200 and 21 Street, Havana 11600, Cuba
  • Juliet Enriquez; Civil Defense National Research Laboratory, Cuba
  • Enrique Noa; Civil Defense National Research Laboratory, Cuba
  • Anamary Suarez; Civil Defense National Research Laboratory, Cuba
  • Cheng Fang; Shanghai Fenglin Glycodrug Promotion Center, Shanghai 200032, China
  • Luis A Espinosa; Center for Genetic Engineering and Biotechnology, Ave 31 e/ 158 y 190, Playa, Havana, Cuba
  • Yassel Ramos; Center for Genetic Engineering and Biotechnology, Ave 31 e/ 158 y 190, Playa, Havana, Cuba
  • Luis Javier Gonzalez; Center for Genetic Engineering and Biotechnology, Ave 31 e/ 158 y 190, Playa, Havana, Cuba
  • Yanet Climent; Finlay Vaccine Institute, 200 and 21 Street, Havana 11600, Cuba.
  • Gertrudis Rojas; Center of Molecular Immunology, P.O. Box 16040, 216 St. Havana, Cuba
  • Ernesto Relova-Hernandez; Center of Molecular Immunology, P.O. Box 16040, 216 St. Havana, Cuba
  • Yanelys Cabrera-Infante; Center of Molecular Immunology, P.O. Box 16040, 216 St. Havana, Cuba
  • Sum Lai Losada; Center of Molecular Immunology, P.O. Box 16040, 216 St. Havana, Cuba
  • Tammy Boggiano; Center of Molecular Immunology, P.O. Box 16040, 216 St. Havana, Cuba
  • Eduardo Ojito; Center of Molecular Immunology, P.O. Box 16040, 216 St. Havana, Cuba
  • Kalet Leon-Monzon; Center of Molecular Immunology, P.O. Box 16040, 216 St. Havana, Cuba
  • Fabrizio Chiodo; Finlay Vaccine Institute, 200 and 21 Street, Havana 11600, Cuba
  • Francoise Paquet; Centre de Biophysique Moleculaire, Centre National de la Recherche Scientifique UPR 4301, rue Charles Sadron, F-45071, Orleans, Cedex 2, France
  • Guangwu Chen; Chengdu Olisynn Biotech. Co. Ltd., and State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu 610041, China.
  • Daniel G Rivera; Laboratory of Synthetic and Biomolecular Chemistry, Faculty of Chemistry, University of Havana, Cuba
  • Dagmar Garcia-Rivera; Finlay Vaccine Institute, 200 and 21 Street, Havana 11600, Cuba
  • Vicente Verez-Bencomo; FinlayVaccine Institute
Preprint en En | PREPRINT-BIORXIV | ID: ppbiorxiv-430146
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ABSTRACT
Controlling the global COVID-19 pandemic depends, among other measures, on developing preventive vaccines at an unprecedented pace. Vaccines approved for use and those in development intend to use neutralizing antibodies to block viral sites binding to the hosts cellular receptors. Virus infection is mediated by the spike glycoprotein trimer on the virion surface via its receptor binding domain (RBD). Antibody response to this domain is an important outcome of the immunization and correlates well with viral neutralization. Here we show that macromolecular constructs with recombinant RBD conjugated to tetanus toxoid induce a potent immune response in laboratory animals. Some advantages of the immunization with the viral antigen coupled to tetanus toxoid have become evident such as predominant IgG immune response due to affinity maturation and long-term specific B-memory cells. This paper demonstrates that subunit conjugate vaccines can be an alternative for COVID-19, paving the way for other viral conjugate vaccines based on the use of small viral proteins involved in the infection process.
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Texto completo: 1 Colección: 09-preprints Base de datos: PREPRINT-BIORXIV Tipo de estudio: Prognostic_studies Idioma: En Año: 2021 Tipo del documento: Preprint
Texto completo
Añadir a Mi BVS
Imprimir
XML
Buscar en Google
Texto completo: 1 Colección: 09-preprints Base de datos: PREPRINT-BIORXIV Tipo de estudio: Prognostic_studies Idioma: En Año: 2021 Tipo del documento: Preprint